白纹伊蚊是一种常见的吸血昆虫，也是登革热、基孔肯雅热等重要传染病的传播媒介。蚊媒的吸血传病主要依赖于嗅觉，但目前对白纹伊蚊嗅觉系统的分子构成和嗅觉发生的分子机制还知之甚少。申请者在前一个国家基金资助下，已对白纹伊蚊的基因组、小RNA组及其与登革病毒感染的关系进行了系统深入研究，在此基础上本项目拟进一步展开下列研究：①克隆鉴定、表达纯化白纹伊蚊的嗅觉结合蛋白OBPs和嗅觉受体ORs基因；②测定OBPs和ORs的时空表达谱及其与嗅觉发生的关系；③解析OBPs和ORs 对气味分子的识别作用及调控机制；④建立白纹伊蚊高效驱避剂和引诱剂的筛选技术平台。研究目标是掌握白纹伊蚊嗅觉系统的分子构成，阐明其嗅觉发生的分子机制，为其驱避剂和引诱剂的研发以及蚊媒传染病的防治提供指南。

Mosquito olfaction is critical to its host-seeking behaviour and thus its ability to reproduce and transmit diseases. There is virtually no information on the molecular mechanisms of olfaction in Aedes albopictus, which is an important emerging disease vector in China. We propose to build on the ongoing collaboration between the Chen laboratory at the Southern Medical University and the Tu laboratory at Virginia Tech to carry out the following specific aims: 1) Characterize A. albopictus OBP and OR genes using molecular cloning; 2) Determine the temporal and spatial expression profile of the OBPs and Ors; 3) Investigate the regulation of OBP and OR genes and their function in odorant detection; 4) Screen for effective repellents and attractants. The overall objectives of the proposal are to illustrate of the molecular mechanisms of olfaction in A. alboptictus and identify potential leads to control vector-borne infectious diseases.