量子点高效电化学发光与核酸分析

电化学发光是当前最先进的生化分析和体外诊断技术之一，而量子点作为一种极富潜力和竞争力的电化学发光探针，引起了研究者浓厚的兴趣。本项目以量子点电化学发光生化分析为导向，拟制备发光性能优异的水溶性量子点，研究不同类型的表面缺陷对量子点电化学发光性能的影响，阐释电化学发光反应路径及机理。研究量子点电化学发光的电子和空穴注入顺序及速率，发展量子点电化学发光信号的电位、光谱和电荷注入动力学辨识方法和体系，构建基于量子点电化学发光的生化分析策略，发展核酸分子高灵敏检测和多种核酸分子同时检测的方法。本项目从量子点的结构设计出发，全面分析量子点电化学发光过程，发展相应的量子点合成方法学和分析体系，将进一步推动量子点电化学发光基础和应用研究。

Electrochemiluminescence (ECL) is one of state-of-the-art biochemical analysis and in vitro diagnosis techniques. Quantum dots have been recognized as one of potential and competitive ECL luminophores and therefore have attracted widespread research interest in recent years. In this project, we plan to synthesize water-soluble quantum dots with high-performance ECL properties for biochemical analysis. The effect of different types of surface trap states will be studied to understand the reaction pathway and mechanism of ECL generation by quantum dots. Then we intend to explore the sequence and kinetics of electron and hole injection to quantum dots for ECL generation. The ultimate goal of this project is to modulate the generation of ECL by quantum dots on the basis of potential, spectrum and charge injection kinetics resolved possibilities, and subsequently develop strategies for highly sensitive nucleic acids detection and simultaneous multiple nucleic acids detection. In summary, the project will focus on the synthesis methods of high-quality quantum dots, thorough study of ECL generation mechanism and their analytical applications. We believe it will bring new insights to understand ECL generation by quantum dots and facilitate their real applications.