基于HIV TRACE研究广西和越南边境地区HIV-1跨境传播的社会-分子网络

广西-越南边境地区艾滋病形势严峻。我们前期流行病学研究显示广西和越南北部存在HIV跨境传播。本项目将利用社会-分子整合网络研究HIV在广西-越南的跨境传播特点。首先，结合前期队列，继续招募研究对象，基于社会网络理论收集信息，绘制研究对象及提名同伴的社会网络；同时收集血液标本，获取HIV pol区和env区序列，加上已有的1139条pol区序列，采用HIV TRACE构建pol区和env区序列的分子网络，运用系统进化分析明确广西-越南跨境传播毒株的演化规律、起源时间、传播方向和强弱；最后，对分子网络和社会网络进行同质性检验，将分子网络的遗传数据映射到社会网络中，构建社会-分子整合网络，并确证基于社会关系而构建的HIV-1传播事件，阐明广西和越南边境HIV-1跨境传播的关键人群、传播地理热点和超级传播链。预期结果将阐明广西-越南HIV跨境传播特征，为该区域精准防控提供科学依据。

The HIV/AIDS epidemic is serous and facing a great challenge in the cross-border areas between Guangxi and Vietnam. Our previous epidemiological studies have found the cross-border HIV transmission between Guangxi and northern Vietnam. This project will highlight the characteristics of cross-border transmission between two areas using social-genetic integration networks. First, we will continue to recruit people living with HIV in the cohort we have established previously for analysis in the cross-border areas, collect epidemiological information of the participates based on the social-network theory, and then map the social relationship between the participates and their nominated peers. At the same time, we will collect blood samples for the participants and identify the sequences of HIV pol and env regions using DNA sequencing approach. The genetic networks of HIV pol and env sequences will be drawn by the HIV TRACE technique, combining with the previous 1139 HIV pol sequences we have collected from the cohort. The evolution dynamic, transmission origin time, and the transmission direction in cross-border areas of different HIV-1 strains between Guangxi and Vietnam will be clarified using the phylogenetic dynamic analysis method. Finally, we will perform homogeneity testing on genetic and social networks separately, and then combine the genetic and social networks to construct a social-genetic integration network, which will confirm HIV-1 transmission events based on social relationships. This project aims to identify the key transmission populations, geographical hot spots of transmission, and super-transmission chains of HIV-1 cross-border transmission between Guangxi and Vietnam. The results of this project expect to provide a scientific basis for the precise prevention and control of HIV-1 cross-border transmission in the Sino-Vietnam border.