基于有向分子-社会传播网络研究广西农村地区老年男性人群HIV-1的传播路径

广西农村地区老年男性艾滋病人数增长较快。我们前期采用普通测序序列进行分子网络研究，发现该人群与女性性工作者存在传播关系，但序列信息有限，只能绘制无向传播分子网络，很难揭示感染者的HIV-1来源和去向以及识别高危传播者。据此，我们拟获取深度测序序列，基于有向分子-社会传播网络研究该区域老年男性人群HIV-1的传播路径。本项目将利用前期建立的队列，以老年男性感染者与女性性工作者作为研究对象，基于社会网络理论收集信息，绘制社会网络；深度测序获取传播个体HIV-1多准种的全长序列，运用可识别个体来源多序列的Phyloscanner进行系统发育分析并绘制有向分子传播网络，最后将有向分子传播网络映射到社会网络，构建整合的有向分子-社会传播网络，阐明老年男性人群HIV-1感染的来源和去向、传播病毒流量、传播关键人群、超级传播链和传播地理热点。本项目研究可为精准防控提供新思路，并为精准干预提供科学依据。

The HIV/AIDS epidemic increased rapidly among elderly males in the rural areas of Guangxi. Our previous molecular network study using sanger sequencing data found that there is a transmission relationship between elderly males and female sex workers. However, due to the limited sequence information of sanger sequencing data, we just got undirected transmission networks, which also make it difficult to reveal the source and sank of HIV-1 among infected persons as well as to accurately identify high-risk transmitters. To solve these problems, we plan to obtain deep sequencing data and study the individualized transmission pathways of HIV-1 among the elderly males in this region based on the directed molecular-social networks. This project will recruit HIV-1-infected elderly males and female sex workers as research subjects from our previously established cohort. Firstly, we will collect social information of all subjects based on social network theory and map social transmission networks. At the same time, we will use the deep sequencing to obtain all of whole sequences of HIV-1 multi-quasispecies within each individual, and use the Phyloscanner software, which can identify multiple sequences from individual sources, to analyze the phylogeny and obtain the directed molecular transmission networks. Finally, the molecular networks will be mapped to the social networks to construct an integrated directed molecular-social networks and to elucidate the HIV-1 transmission source and sank, Virus transmission scores, high-risk transmission groups, transmission geographic hotpots as well as super transmission chains among the elderly males. This project will provide a new method for precise prevention and control, and provide scientific basis for precise intervention of HIV/AIDS epidemic among elderly males in rural areas of Guangxi.