基于微米尺度暂态离子传输的活体分析研究

活体电分析化学是分析化学和神经科学高度交叉且充满挑战的前沿研究领域之一，发展新的活体电化学分析原理和方法对于理解和认识生理病理过程中的分子机制具有重要的意义。本项目拟在申请者建立微米管整流模型，并结合在离子传输方面的研究基础，通过内壁修饰调控离子传输，发展基于微米管整流的分析新原理和新方法，并探索方法在活体分析中的适用性。研究内容包括：1）生理活性物质（如ATP，H+)的高选择性界面设计；2）功能化微米管分析新原理和新方法的建立；3）高时空分辨分析原理和方法的建立；4）方法在活体分析中的适用性研究。本项目的实施不仅可以促进活体分析化学新原理、新技术和新方法的发展，而且也将有效推进分析化学和神经科学的深度交叉和融合。

In Vivo electroanalytical chemistry is a highly interdisciplinary and challenging research field between analytical chemistry and neuroscience. Developing new principles and methods for in vivo analysis is highly important to understand the molecular mechanism in the physiological and pathological process. Based on our previous work of ion current rectification in microscale and ion transport in nanopores, we would regulate the ion transport through the functional modification on the pipette inner walls and develop new analytical technique for in vivo analysis. The main research includes the following aspects: 1) design high selectivity interface for physiological active substances (such as ATP and H+); 2) establish new analytical principle and methods based on functional micropipette; 3) develop high spatial and temporal resolution analysis; 4) explore the applicability of methods for in vivo analysis. This project will not only promote the development of new principles and methods for in vivo analysis, but also effectively facilitate the integration of analytical chemistry and neuroscience.