我们前期研究提示间充质干细胞(MSC)来源的微泡(MV)具有肾损伤修复作用，同时我们在MSC-MV中检出276种miRNA，其中包括含量较高的miR-200家族。最新研究显示miR-200可以直接靶向抑制Map3k1, Mapk12, Snail1基因的表达，从而发挥纤维化的抑制作用。本项目在前期研究基础上，进一步摸索MSC-MV抑制肾脏纤维化的机制及其相关信号通路。主要研究内容为：(1)证明MSC-MV通过传递miR-200发挥抑制肾脏纤维化的作用；(2)探明MSC-MV是否通过传递miR-200直接靶向MAPK-snail通路而逆转肾间质纤维化。本项目将为探索干细胞治疗慢性肾脏纤维化的新方法提供进一步的理论依据。

Our previous study found that the microvesicles derived from mesenchymal stem cells (MSC-MV) had the effect in repairing renal injury. Meanwhile, more than 276 kinds of miRNA including the relative high content of miR-200 family were detected in MSC-MV. Recently study demonstrated that miR-200 could directly inhibit the gene expression of Map3k1, Mapk12 and Snail1 to attenuate renal fibrosis. Based on the previous research, this aim of these project is to further investigate the possible mechanisms and signal pathway in inhibiting renal fibrosis by MSC-MV. This study will focus on:(1)exploring the function of miR-200 delivered by MSC-MV in the renal restoration of kidney injury; (2) determining whether miR-200 delivered by MSC-MV could directly inhibit MAPK-snail pathway to repair renal fibrosis . This project will provide further theoretical basis for the exploration of new methods of stem cell therapy for renal fibrosis.