Natural rodent model of viral transmission reveals biological features of virus population dynamics.
Natural rodent model of viral transmission reveals biological features of virus population dynamics.
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DOI：
10.1084/jem.20211220
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发表时间：
2022-02-07
期刊：
影响因子：
--
通讯作者：
Langlois RA
中科院分区：
文献类型：
--
作者：
Fay EJ;Balla KM;Roach SN;Shepherd FK;Putri DS;Wiggen TD;Goldstein SA;Pierson MJ;Ferris MT;Thefaine CE;Tucker A;Salnikov M;Cortez V;Compton SR;Kotenko SV;Hunter RC;Masopust D;Elde NC;Langlois RA
病毒种类繁多，无处不在，但许多感染模式并不能概括自然传播和进化。在这里，我们连接自然和实验系统，研究宿主内和宿主之间的实时传播动态，识别新型病毒，并推断病毒-病原体关系。新出现的病毒威胁着全球健康，但很少有实验模型可以描述病毒和跨物种传播所需的宿主因素。在这里，我们利用了一个模型，即宠物店的老鼠或老鼠，其中港口天然啮齿类病原体，是共同圈养与实验室小鼠。这种“脏”小鼠模型为研究病毒通过自然机制在宿主之间和宿主内的急性传播提供了平台。我们确定了许多病毒和其他微生物物种，传播到共同圈养的小鼠，包括冠状病毒科，星状病毒科，小核糖核酸病毒科和Narnaviridae家族的潜在新成员，并发现了促进或阻止病毒传播的病原体相互作用。我们还评估了小鼠星状病毒在新宿主中传播和扩散的传播动力学。最后，通过将我们的实验室小鼠与宠物店大鼠的垫料共同饲养，我们确定了大鼠星状病毒的跨物种传播。总体而言，该模型系统允许分析天然啮齿动物病毒的传播，是进一步表征人畜共患病屏障的平台。
Viruses are diverse and ubiquitous, but many infection models do not recapitulate natural transmission and evolution. Here we bridge natural and experimental systems to study real-time transmission dynamics within and between hosts, identify novel viruses, and infer virus–pathogen relationships. Emerging viruses threaten global health, but few experimental models can characterize the virus and host factors necessary for within- and cross-species transmission. Here, we leverage a model whereby pet store mice or rats—which harbor natural rodent pathogens—are cohoused with laboratory mice. This “dirty” mouse model offers a platform for studying acute transmission of viruses between and within hosts via natural mechanisms. We identified numerous viruses and other microbial species that transmit to cohoused mice, including prospective new members of the Coronaviridae, Astroviridae, Picornaviridae, and Narnaviridae families, and uncovered pathogen interactions that promote or prevent virus transmission. We also evaluated transmission dynamics of murine astroviruses during transmission and spread within a new host. Finally, by cohousing our laboratory mice with the bedding of pet store rats, we identified cross-species transmission of a rat astrovirus. Overall, this model system allows for the analysis of transmission of natural rodent viruses and is a platform to further characterize barriers to zoonosis.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
校正的肌肉校准力精度在啮齿动物的分子树中精确的放置。
影响因子：
4.6
作者：
Kimura Y;Hawkins MT;McDonough MM;Jacobs LL;Flynn LJ
通讯作者：
Flynn LJ
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.7
作者：
Gralinski, Lisa E.;Menachery, Vineet D.
通讯作者：
Menachery, Vineet D.
DOI：
10.1056/nejmoa2105000
发表时间：
2021-06-10
期刊：
The New England journal of medicine
影响因子：
--
作者：
Hacisuleyman E;Hale C;Saito Y;Blachere NE;Bergh M;Conlon EG;Schaefer-Babajew DJ;DaSilva J;Muecksch F;Gaebler C;Lifton R;Nussenzweig MC;Hatziioannou T;Bieniasz PD;Darnell RB
通讯作者：
Darnell RB