Evaluation of the site-unspecified peptide identification method for proteolytic peptide mapping.
Evaluation of the site-unspecified peptide identification method for proteolytic peptide mapping.
复制标题
蛋白水解肽图谱中非特定位点肽鉴定方法的评价
作者：
蛋白酶在食品工业中广泛用于水解蛋白质和制备生物活性肽。肽图谱鉴定支持蛋白酶在食品工业中的应用。针对胰蛋白酶等位点特异性蛋白酶开发的位点特异性肽鉴定方法相对成熟可靠，但不能应用于大多数位点特异性较弱的工业蛋白酶。为了解决这一问题，应调查和评估非特定位点肽鉴定方法的性能和可靠性。在这项研究中，单一蛋白和蛋白混合物的胰蛋白酶水解产物被用来评估非特定位点鉴别方法。在数据库搜索中没有指定水解蛋白的物种来源，这意味着包括数百万个蛋白质序列用于计算和匹配。至少98%的胰蛋白酶肽通过非特定位点方法被成功鉴定，表明非特定位点方法显示出有希望的可靠性。此外，位点非特异性方法比位点特异性方法鉴定了更多的肽，包括来自胰蛋白酶的低频位点非特异性水解的肽，表明该方法具有较强的肽图谱能力。结果表明，非位点肽鉴定方法在非位点工业蛋白酶研究中的适用性。
Proteases are widely used in the food industry to hydrolyze proteins and prepare bioactive peptides. Peptide mapping identification supports the application of proteases in the food industry. The site-specified peptide identification method, which was developed for site-specific proteases like trypsin, is relatively mature and reliable but cannot be applied using most industrial proteases with weak site specificity. To address this issue, the performance and reliability of the site-unspecified peptide identification method should be investigated and evaluated. In this study, tryptic hydrolysates of a single protein and a protein mixture were used to evaluate the site-unspecified identification method. The species origin of the hydrolyzed proteins was not specified in a database search, meaning that millions of protein sequences were included for calculating and matching. At least 98% of the tryptic peptides were successfully identified via the site-unspecified method, demonstrating that the site-unspecified method shows promising reliability. Moreover, the site-unspecified method identified more peptides than the site-specified method, including those from the low-frequency site-unspecific hydrolysis of trypsin, suggesting that the method has strong capabilities for peptide mapping. The results indicate the applicability of the site-unspecified peptide identification method in the study of site-unspecific industrial proteases.
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影响因子：
16.6
作者：
The, Matthew;Kall, Lukas
通讯作者：
Kall, Lukas
影响因子：
6.4
作者：
Zhou, Cuixia;Zhou, Huiying;Lu, Fuping
通讯作者：
Lu, Fuping
影响因子：
3.3
作者：
Wang, Hongbin;Wang, Jing;Lu, Fuping
通讯作者：
Lu, Fuping
DOI：
10.1007/978-1-4939-3167-5_2
发表时间：
2016-01-01
期刊：
PLANT BIOINFORMATICS: METHODS AND PROTOCOLS, 2ND EDITION
影响因子：
--
作者：
Boutet, Emmanuel;Lieberherr, Damien;Xenarios, Ioannis
通讯作者：
Xenarios, Ioannis
影响因子：
4.2
作者：
Chen, Ying-Lan;Chang, Wei-Hung;Chen, Yet-Ran
通讯作者：
Chen, Yet-Ran