Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency.
Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency.
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DOI：
10.3390/cimb43020081
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发表时间：
2021-09-13
影响因子：
3.1
通讯作者：
Gao S
中科院分区：
文献类型：
--
作者：
Nie Z;Luo B;Zhang X;Wu L;Liu D;Guo J;He X;Gao D;Gao S;Gao S
大量营养元素磷酸盐（Pi）的缺乏会导致植物在形态、生理和分子水平上发生各种变化。然而，对低磷条件下调节磷稳态的分子机制仍然知之甚少，特别是在玉米中，这是一种主要作物，需要大量的Pi。因此，在本研究中，我们使用RNA测序和同量异位素标签进行相对和绝对定量（iTRAQ），在转录组和蛋白质组水平上对具有Pi耐受基因型的玉米幼苗的茎和根进行表达谱分析。我们确定了1944差异表达的转录本和340差异表达的蛋白质在低磷条件下。大多数差异表达的基因被聚类为调节因子，例如在转录水平上参与Pi信号通路的转录因子。然而，更多的功能和代谢相关的基因表现出蛋白质水平的表达变化。此外，在低磷条件下，磷转运蛋白和磷酸酶在转录和蛋白质水平上的根中被特异性地诱导，并且在根中特异性地检测到两种紫色酸性磷酸酶（PAP）和一种UDP-磺基喹诺糖合酶（SQD）的mRNA和蛋白质的量增加。本研究为提高玉米磷素利用效率提供了新的思路。
A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ). We identified 1944 differentially expressed transcripts and 340 differentially expressed proteins under low-Pi conditions. Most of the differentially expressed genes were clustered as regulators, such as transcription factors involved in the Pi signaling pathway at the transcript level. However, the more functional and metabolism-related genes showed expression changes at the protein level. Moreover, under low-Pi conditions, Pi transporters and phosphatases were specifically induced in the roots at both the transcript and protein levels, and increased amounts of mRNA and protein of two purple acid phosphatases (PAPs) and one UDP-sulfoquinovose synthase (SQD) were specifically detected in the roots. The new insights provided by this study will help to improve the P-utilization efficiency of maize.
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影响因子：
11.6
作者：
Casimiro, I;Marchant, A;Bennett, M
通讯作者：
Bennett, M
PlantTFDB 4.0：成为植物转录因子和调控相互作用的中心枢纽。
影响因子：
14.9
作者：
Jin J;Tian F;Yang DC;Meng YQ;Kong L;Luo J;Gao G
通讯作者：
Gao G
DOI：
10.1073/pnas.95.4.1950
发表时间：
1998-02-17
影响因子：
11.1
作者：
Essigmann, B;Güler, S;Benning, C
通讯作者：
Benning, C
影响因子：
7
作者：
Lan, Ping;Li, Wenfeng;Schmidt, Wolfgang
通讯作者：
Schmidt, Wolfgang
影响因子：
27.5
作者：
Cubero, Beatriz;Nakagawa, Yuko;Pardo, Jose M.
通讯作者：
Pardo, Jose M.