Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages.
Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages.
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DOI：
10.1039/c5nr01857a
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发表时间：
2015-06-14
期刊：
影响因子：
6.7
通讯作者：
Chiu DT
中科院分区：
文献类型：
--
作者：
Ye F;White CC;Jin Y;Hu X;Hayden S;Zhang X;Gao X;Kavanagh TJ;Chiu DT
PDot生物应用的快速开发和接受取决于对其细胞毒性的深入了解。在本文中，我们在细胞效应水平（如细胞活力，增殖和坏死）和更微妙的影响（如氧化还原应激）对RAW264.7细胞（一种与体内纳米颗粒分布高度相关的小鼠巨噬细胞系）进行了全面的PDot细胞毒性研究。评估的氧化还原应激测量值为线粒体内膜脂质过氧化（壬基吖啶橙子，NAO）、总巯基水平（单溴异丙亚胺，MBB）和吡啶核苷酸氧化还原状态（NAD（P）H自体荧光）。由于已经用QDots对纳米毒性进行了大量的研究，也由于它们的尺寸相当，因此选择QDots作为本研究的比较/参考纳米颗粒。结果表明，PDots表现出的细胞毒性作用比它们的无机类似物（QDots）小得多，并且更亮，允许在各种生物应用中使用低得多的浓度。此外，在较低剂量水平（2.5 nM至10 nM）下，PDot处理导致总巯基水平高于QDots。在更高的剂量水平（20 nM至40 nM）下，QDots在RAW 264.7细胞中引起的硫醇水平显著高于PDots，表明QDots通过上调GSH合成引起对氧化应激的补偿。在较高浓度的QDots下，NAD（P）H水平显示出初始消耗，然后补充至高于媒介物对照的水平。PDot显示出类似的趋势，但这在统计学上不显著。因为PDot在低浓度下比QDot引起更少的氧化应激和细胞毒性，并且因为它们在这些低浓度下表现出上级荧光，所以预测PDot在生物医学应用中具有增强的实用性。
The rapid development and acceptance of PDots for biological applications depends on an in depth understanding of their cytotoxicity. In this paper, we performed a comprehensive study of PDot cytotoxicity at both the gross cell effect level (such as cell viability, proliferation and necrosis) and more subtle effects (such as redox stress) on RAW264.7 cells, a murine macrophage cell line with high relevance to in vivo nanoparticle disposition. The redox stress measurements assessed were inner mitochondrial membrane lipid peroxidation (nonyl-acridine orange, NAO), total thiol level (monobromobimane, MBB), and pyridine nucleotide redox status (NAD(P)H autofluorescence). Because of the extensive work already performed with QDots on nanotoxicity and also because of their comparable size, QDots were chosen as a comparison/reference nanoparticle for this study. The results showed that PDots exhibit cytotoxic effects to a much lesser degree than their inorganic analogue (QDots) and are much brighter, allowing for much lower concentrations to be used in various biological applications. In addition, at lower dose levels (2.5 nM to 10 nM) PDot treatment resulted in higher total thiol level than those found with QDots. At higher dose levels (20 nM to 40 nM) QDots caused significantly higher thiol levels in RAW264.7 cells, than was seen with PDots, suggesting that QDots elicit compensation to oxidative stress by upregulating GSH synthesis. At the higher concentrations of QDots, NAD(P)H levels showed an initial depletion, then repletion to a level that was greater than vehicle controls. PDots showed a similar trend but this was not statistically significant. Because PDots elicit less oxidative stress and cytotoxicity at low concentrations than QDots, and because they exhibit superior fluorescence at these low concentrations, PDots are predicted to have enhanced utility in biomedical applications.
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影响因子：
38.3
作者：
通讯作者：
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影响因子：
13.3
作者：
Geng, Junlong;Sun, Chunyang;Liu, Bin
通讯作者：
Liu, Bin
影响因子：
7.4
作者：
Sun, Wei;Ye, Fangmao;Gallina, Maria E.;Yu, Jiangbo;Wu, Changfeng;Chiu, Daniel T.
通讯作者：
Chiu, Daniel T.
DOI：
10.1039/c2cc16486h
发表时间：
2012-02-07
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Ye F;Wu C;Jin Y;Wang M;Chan YH;Yu J;Sun W;Hayden S;Chiu DT
通讯作者：
Chiu DT
影响因子：
14
作者：
Soenen, Stefaan J.;Demeester, Jo;Braeckmans, Kevin
通讯作者：
Braeckmans, Kevin