Transferrin as a model system for method development to study structure, dynamics and interactions of metalloproteins using mass spectrometry.
Transferrin as a model system for method development to study structure, dynamics and interactions of metalloproteins using mass spectrometry.
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DOI：
10.1016/j.bbagen.2011.06.019
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发表时间：
2012-03
影响因子：
3
通讯作者：
Gumerov, Dmitry R.
中科院分区：
文献类型：
--
作者：
Kaltashov, Igor A.;Bobst, Cedric E.;Zhang, Mingxuan;Leverence, Rachael;Gumerov, Dmitry R.
关键词：
转铁蛋白（Transferrin，Tf）是一种典型的金属蛋白，由于其在铁稳态中的独特作用和作为广泛治疗的组成部分的巨大前景，在过去已经被广泛研究，并且仍然是许多研究工作的焦点。电喷雾离子化质谱（ESI MS）是一种有效的分析工具，其已成功地用于研究Tf和基于Tf的产物的各种性质，范围从共价结构和金属结合到构象以及与其生理伴侣的相互作用。各种基于ESI MS的技术产生关于Tf性质和行为的独特信息，这些信息与其他实验技术提供的信息高度互补。为Tf研究开发的基于实验ESI MS的技术不仅有助于了解这种蛋白质的铁结合特性的基本方面和优化基于Tf的治疗产品，而且还可以应用于研究一系列其他金属蛋白。
Transferrin (Tf) is a paradigmatic metalloprotein, which has been extensively studied in the past and still is a focal point of numerous investigation efforts owing to its unique role in iron homeostasis and enormous promise as a component of a wide range of therapies. Electrospray ionization mass spectrometry (ESI MS) is a potent analytical tool that has been used successfully to study various properties of Tf and Tf-based products, ranging from covalent structure and metal binding to conformation and interaction with their physiological partners. Various ESI MS-based techniques produce unique information on Tf properties and behavior that is highly complementary to information provided by other experimental techniques. The experimental ESI MS-based techniques developed for Tf studies are not only useful for understanding of fundamental aspects of the iron-binding properties of this protein and optimizing Tf-based therapeutic products, but can also be applied to study a range of other metalloproteins.
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影响因子：
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作者：
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DOI：
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