Microfabricated flow system for ammonia and creatinine with an air-gap structure
Microfabricated flow system for ammonia and creatinine with an air-gap structure
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具有气隙结构的氨和肌酐微加工流动系统
DOI：
10.1016/j.snb.2003.08.018
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发表时间：
2004
影响因子：
8.4
通讯作者：
Yasuaki Matsugi
中科院分区：
文献类型：
--
作者：
H. Suzuki;Yasuaki Matsugi
利用在流动通道中形成的空气间隙，研制了一种快速响应的Severinghaus型氨气敏电极。用于氨气感测电极的电解质溶液与流动通道中的样品溶液通过由插入在电极基板和流动通道之间的硅橡胶的疏水层保持的气隙分离。当样品溶液被引入流动通道时，氨通过气隙消散到电解质溶液中。用薄膜pH指示电极测量伴随的pH变化。由于气隙，响应明显快于传统结构的电极。当氨浓度高于1mM时，90%响应时间小于1min。线性关系良好，检测下限为20μM。用氨电极构建肌酸酐传感系统。当溶液pH为10.0时，检测下限为50μM，90%响应时间为4min。采用双指示电极结构，采用差分信号，可以最大限度地减少内源氨的影响。在存在浓度相当的氨的情况下，可以清楚地区分源自肌酸酐的信号。
A fast-responding Severinghaus-type ammonia gas-sensing electrode was developed by using an air gap formed in a flow channel. An electrolyte solution for the ammonia gas-sensing electrode was separated from a sample solution in the flow channel by an air gap maintained with a hydrophobic layer of silicone rubber intercalated between the electrode substrate and the flow channel. When a sample solution is introduced into the flow channel, ammonia dissipates through the air gap into the electrolyte solution. The accompanying pH change was measured with a thin-film pH-indicator electrode. Because of the air gap, the response was significantly faster than the electrodes of the conventional structure. The 90% response time was less than 1min for an ammonia concentration higher than 1mM. A linear relationship was observed in the calibration curve, and the lower detection limit was 20μM. The ammonia electrode was used to construct a creatinine-sensing system. When the solution pH was 10.0, the lower detection limit was 50μM, and the 90% response time was 4min around the detection limit. The influence of endogenous ammonia could be minimized by taking a differential signal with a double indicator electrode structure. The signal originating from creatinine could be differentiated clearly in the presence of ammonia of comparable concentrations.
用于自动酶分析和电位氨检测的在线气体透析器。
影响因子：
7.4
作者：
Fraticelli,YM;Meyerhoff,ME
通讯作者：
Meyerhoff,ME