Development and Characterization of Transcription Factor Gene-Derived Microsatellite (TFGM) Markers in Medicago truncatula and Their Transferability in Leguminous and Non-Leguminous Species.
Development and Characterization of Transcription Factor Gene-Derived Microsatellite (TFGM) Markers in Medicago truncatula and Their Transferability in Leguminous and Non-Leguminous Species.
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DOI：
10.3390/molecules20058759
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发表时间：
2015-05-15
期刊：
影响因子：
--
通讯作者：
Xie W
中科院分区：
文献类型：
--
作者：
Liu W;Jia X;Liu Z;Zhang Z;Wang Y;Liu Z;Xie W
转录因子 (TF) 是关键的衔接分子，通过控制基因表达来调节许多植物过程。最近 TF 数据可用性的增加使 TF 成为基因功能微卫星标记开发的宝贵资源。在本研究中，我们为蒺藜苜蓿开发了 TF 基因衍生的微卫星 (TFGM) 标记，并评估了其跨物种可转移性。从 1467 个蒺藜 TF 编码序列中总共鉴定出 203 个 SSR，其中 87.68% 是三核苷酸重复，其次是单核苷酸重复（4.93%）和六核苷酸重复（1.48%）。此外，142 个 TFGM 标记显示出对豆科 (55.63%–85.21%) 和非豆科 (28.17%–50.00%) 物种的高水平可转移性。在 44 个苜蓿种质中评估了 27 个 TFGM 标记的多态性。每个标记的等位基因数范围为 2 至 8 个，平均为 4.41，PIC 值范围为 0.08 至 0.84，平均为 0.60。考虑到高度多态性，我们研究中开发的这些 TFGM 标记对于豆科和非豆科物种的遗传关系评估、标记辅助选择和比较基因组研究具有重要价值。
Transcription factors (TFs) are critical adaptor molecules that regulate many plant processes by controlling gene expression. The recent increase in the availability of TF data has made TFs a valuable resource for genic functional microsatellite marker development. In the present study, we developed TF gene-derived microsatellite (TFGM) markers for Medicago truncatula and assessed their cross-species transferability. A total of 203 SSRs were identified from 1467 M. truncatula TF coding sequences, 87.68% of which were trinucleotide repeats, followed by mono- (4.93%) and hexanucleotide repeats (1.48%). Further, 142 TFGM markers showed a high level of transferability to the leguminous (55.63%–85.21%) and non-leguminous (28.17%–50.00%) species. Polymorphisms of 27 TFGM markers were evaluated in 44 alfalfa accessions. The allele number per marker ranged from two to eight with an average of 4.41, and the PIC values ranged from 0.08 to 0.84 with an average of 0.60. Considering the high polymorphism, these TFGM markers developed in our study will be valuable for genetic relationship assessments, marker-assisted selection and comparative genomic studies in leguminous and non-leguminous species.
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