Egg-grown and tissue-culture-grown variants of influenza A (H3N2) virus with special attention to their use as antigens in seroepidemiology
Egg-grown and tissue-culture-grown variants of influenza A (H3N2) virus with special attention to their use as antigens in seroepidemiology
复制标题
甲型流感 (H3N2) 病毒的鸡蛋生长和组织培养变种，特别注意它们在血清流行病学中作为抗原的用途
作者：
R. Pyhälä;L. Pyhälä;M. Valle;K. Aho
对1984-5年流感暴发（A/Finland/13/85E）期间从胚胎蛋中分离到的一株甲型流感（H3N2）病毒与从MDCK细胞培养（A/Finland/13/85M）中分离到的同一临床标本中的病毒进行了抗原分析比较。m病毒对抗异源病毒的血凝抑制抗体似乎比病毒更敏感。繁殖和斑块纯化实验的结果支持这样的假设，即单个临床标本可能由宿主在分离过程中选择性促进的不同抗原变异亚群组成。本文讨论了受体结合特性作为这种选择性的可能解释。对1984 - 1985年同一研究对象的流行前和流行后标本471对血清进行血凝抑制抗体检测，检测6种甲型（H3N2）流感病毒株，包括来自A/Finland/13/85的e-病毒和m -病毒。在所使用的抗原中，m病毒检测到的显著抗体增加频率高于e病毒（10.0 vs . 5.9%）。m病毒的优势可能主要在于它能够挑选出遗忘性抗体反应。不考虑这种交叉反应性，对m病毒的流行前抗体与保护作用相当相关。在1985年夏季采集的230份不同年龄组的血清中，m -病毒抗体状态明显优于e -病毒抗体状态。结果表明，至少在某些情况下，针对mdck生长的病毒的抗体比针对鸡蛋生长的病毒变体的抗体更准确地指示了社区的免疫状态。
SUMMARY A field strain of influenza A (H3N2) virus isolated in embryonated eggs during the 1984–5 influenza outbreak (A/Finland/13/85E) was compared in an antigenic analysis with virus from the same clinical specimen isolated in MDCK cell cultures (A/Finland/13/85M). The M-virus appeared to be more sensitive to haemagglutination-inhibiting antibodies against heterologous viruses than did the Evirus. The results of propagation and plaque purification experiments support the hypothesis that a single clinical specimen may consist of distinct antigenic variant subpopulations promoted selectively by the host during isolation procedures. Receptor-binding properties are discussed as a possible explanation for this selectivity. A set of 471 paired sera consisting of pre-epidemic and post-epidemic specimens taken from the same subjects in 1984–5 was studied for haemagglutinationinhibiting antibodies to six influenza A (H3N2) virus strains, including the E-virus and the M-virus from A/Finland/13/85. Of the antigens used, the M-virus detected significant antibody increases more frequently than did the E-virus (10·0 v. 5·9%). The superiority of the M-virus may rest primarily in its ability to pick out anamnestic antibody responses. Irrespective of this cross-reactivity, preepidemic antibody to the M-virus was fairly well associated with protection. In the set of sera (230 specimens) collected in summer 1985 to represent different age groups, the antibody status against the M-virus was significantly better than the status against the E-virus. The results suggest that, at least in some instances, antibody to MDCK-grown virus is a more accurate indicator of the immune status of a community than antibodies to egg-grown virus variants.
影响因子：
3.7
作者：
ROGERS, GN;PRITCHETT, TJ;PAULSON, JC
通讯作者：
PAULSON, JC
抗原相似的流感病毒血凝素的不同细胞表面受体决定簇。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Carroll,SM;Higa,HH;Paulson,JC
通讯作者：
Paulson,JC
影响因子：
3.7
作者：
ROGERS, GN;PAULSON, JC
通讯作者：
PAULSON, JC