Differential expression of calcium-binding proteins in the red nucleus of the developing and adult human brain
Differential expression of calcium-binding proteins in the red nucleus of the developing and adult human brain
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发育中和成人大脑红核中钙结合蛋白的差异表达
DOI：
10.1007/s004290000147
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Wood
中科院分区：
文献类型：
--
作者：
N. Ulfig;Wood
成人红核由两部分组成：（1）小细胞部分，与（2）大细胞部分明显分开。已知后者及其红核脊髓投射在成人大脑中处于初级阶段。有关胎儿或新生儿红核特征的信息很少。本研究旨在详细说明三种钙结合蛋白的分布：钙结合蛋白 (CR)、钙结合蛋白 (CB) 和小白蛋白 (PV)，已知这三种钙结合蛋白在不同的神经元群体中表达。特别关注瞬态现象。 CB是胎儿和围产期大脑大细胞部分最丰富的蛋白质；免疫反应（ir）神经元出现数量众多且密集。在成人体内，仅存在少量且间隔较远的神经细胞。 CR 表达很大程度上与 CB 一致，只是免疫标记的神经元较少。在双标记中，大多数神经元同时表达 CB 和 CR；大细胞部分存在中等数量的仅表达CR的神经细胞。在胎儿、围产期以及成人的细小细胞部分中观察到PV-ir纤维和中等数量的小细胞。在胎儿和围产期大脑的大细胞部分中发现了一些 PV-ir 神经元。我们的结果表明：（1）红核的大细胞部分和细细胞部分从胎儿期开始就是界限分明的部分，因此在发育过程中细细胞部分相对于大细胞部分占主导地位； (2)大细胞部分在胎儿期比成年期更为突出； (3) 红核中的神经元对所检查的三种钙结合蛋白的免疫反应性是异质的； (4) 大细胞部分的短暂突出可能是运动行为的特定短暂模式的基础。
The adult human red nucleus consists of two parts: (1) the parvocellular part, which is clearly separated from (2) the magnocellular part. The latter and its rubrospinal projection is known to be rudimentary in the adult human brain. Information concerning the fetal or neonatal features of the red nucleus is sparse. This study is aimed at providing a detailed account of the distribution of three calcium-binding proteins: calretinin (CR), calbindin (CB), and parvalbumin (PV), which are known to be expressed in distinct neuronal populations. Special attention has been paid to transient phenomena. CB was the most abundant protein in the magnocellular part in fetal and perinatal brains; immunoreactive (ir) neurons appeared numerous and densely packed. In the adult only few and widely spaced ir nerve cells were present. CR-expression largely corresponds to that of CB, except that fewer neurons were immunolabelled. In double- labellings the majority of neurons expressed both CB and CR; a moderate number of nerve cells solely expressing CR was present in the magnocellular part. PV-ir fibers and a moderate number of small cells were observed in the fetal, perinatal as well as the adult parvocellular part. A few PV-ir neurons were seen in the magnocellular part of the fetal and perinatal brains. Our results indicated that: (1) the magnocellular and parvocellular parts of the red nucleus were well-demarcated portions from fetal life onwards, thus a dominance of the parvocellular part over the magnocellular occurred during development; (2) the magnocellular part was more prominent in the fetal period than in adulthood; (3) neurons in the red nucleus were heterogeneous with respect to the immunoreactivities towards the three calcium-binding proteins examined; (4) the transient prominence of the magnocellular part might be a substrate for a specific transitory pattern of motor behaviour.
影响因子：
56.9
作者：
GHOSH, A;CARNAHAN, J;GREENBERG, ME
通讯作者：
GREENBERG, ME