Role of immune-related lncRNAs--PRKCQ-AS1 and EGOT in the regulation of IL-1β, IL-6 and IL-8 expression in human gingival fibroblasts with TNF-α stimulation.
Role of immune-related lncRNAs--PRKCQ-AS1 and EGOT in the regulation of IL-1β, IL-6 and IL-8 expression in human gingival fibroblasts with TNF-α stimulation.
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免疫相关 lncRNAs--PRKCQ-AS1 和 EGOT 在 TNF-α 刺激下调节人牙龈成纤维细胞 IL-1β、IL-6 和 IL-8 表达中的作用
DOI：
10.1016/j.jds.2022.06.006
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发表时间：
2023-01
影响因子：
3.5
通讯作者：
Zhong, Jinsheng
中科院分区：
文献类型：
--
作者：
Zhao, Quanquan;Liu, Jianru;Ouyang, Xiangying;Liu, Wenyi;Lv, Peiying;Zhang, Shengnan;Zhong, Jinsheng
已有研究表明lncRNA对免疫相关疾病有一定的影响，但其在牙周炎中的作用还有待进一步研究。本研究旨在寻找免疫相关lncRNA在牙周炎中的表达，并初步探讨其在牙周炎中的作用。CIBERSORT法检测牙周组织中免疫细胞的丰度。计算lncRNA的表达谱与免疫细胞丰度之间的相关性，并鉴定免疫相关的lncRNA。采用RT-qPCR方法检测15例牙周炎组织和15例健康牙龈组织中免疫相关lncRNA的表达。检测不同浓度TNF-α（0、10、15、20、30 ng/mL）刺激HGFs不同时间（0、12、24、48 h）后PRKCQ AS 1和EGOT的表达。然后，使用siRNA沉默HGF中的PRKCQ-AS 1和EGOT。用15 ng/mL TNF-α刺激HGFs，观察HGFs表达IL-1β、IL-6、IL-8的水平，以及NF-κB通路的激活情况。PRKCQ-AS 1和EGOT被鉴定为牙周组织中前2位的免疫相关lncRNA。PRKCQ-AS 1和EGOT在炎症牙周组织和TNF-α刺激的HGFs中的表达显著上调。PRKCQ-AS 1和EGOT基因敲低后，TNF-α刺激的HGFs中IL-1β、IL-6和IL-8的表达水平降低，NF-κB通路的激活受到抑制。PRKCQ-AS 1和EGOT是牙周组织中最早发现的免疫相关lncRNAs，它们通过NF-κB通路调控HGF的IL-1β、IL-6和IL-8的表达。
It was reported that lncRNAs have an effect on immune-related diseases, however, their roles in periodontitis remain to be investigated. The aim of this study was to look for immune-related lncRNAs in periodontitis, and to preliminarily explore their function in vitro. CIBERSORT was used to analyze abundance of immune cell in the periodontal tissue. Correlation between the expression profile of lncRNAs and abundance of immune cell was calculated and immune-related lncRNAs were identified. The expressions of immune-related lncRNAs identified were validated by RT-qPCR with 15 periodontitis and 15 healthy gingival tissues. The expressions of PRKCQ-AS1 and EGOT in HGFs were detected under the stimulation of different concentrations of TNF-α (0, 10, 15, 20, 30 ng/mL) and different duration (0, 12, 24 and 48 h). Then, siRNA was used to silence PRKCQ-AS1 and EGOT in HGFs. The expression level of IL-1β, IL-6, IL-8 of the HGFs after stimulated by 15 ng/mL TNF-α, and the activation of NF-κB pathway was observed. PRKCQ-AS1 and EGOT were identified as top 2 immune-related lncRNAs in periodontal tissues. The expressions of PRKCQ-AS1 and EGOT were significantly up-regulated in inflamed periodontal tissue and in HGFs under TNF-α stimulation. After knock-down of PRKCQ-AS1 and EGOT, expression level of IL-1β, IL-6, and IL-8 in HGFs with TNF-α stimulation were decreased, and activation of NF-κB pathway was inhibited. PRKCQ-AS1 and EGOT were firstly identified as immune-related lncRNAs in periodontal tissue, and they regulate the expression of IL-1β, IL-6, and IL-8 of HGFs through the NF-κB pathway.
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DOI：
10.1165/ajrcmb.15.1.8679226
发表时间：
1996-07-01
影响因子：
6.4
作者：
Nicholson, WJ;Slight, J;Donaldson, K
通讯作者：
Donaldson, K
Rho 激酶抑制剂 Y-27632 通过阻断人牙龈成纤维细胞中的 p38 MAPK 和 NF-kappa B 途径下调 LPS 诱导的 IL-6 和 IL-8 产生
影响因子：
4.3
作者：
Kang, Wenyan;Shang, Lingling;Ge, Shaohua
通讯作者：
Ge, Shaohua
影响因子：
3.8
作者：
Kawamoto, Dione;Lucas Amado, Pamela Pontes;Alves Mayer, Marcia Pinto
通讯作者：
Alves Mayer, Marcia Pinto
通过加权基因共表达网络分析鉴定与牙周炎相关的新型关键lncRNA
影响因子：
3.5
作者：
Jin, Su-Han;Zhou, Rui-Hao;Liu, Jian-Guo
通讯作者：
Liu, Jian-Guo
影响因子：
5.1
作者：
Liu, Jianru;Wang, Yixiang;Ouyang, Xiangying
通讯作者：
Ouyang, Xiangying