Relationship between HOX gene and pediatric congenital clubfoot.
Relationship between HOX gene and pediatric congenital clubfoot.
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DOI：
10.3892/etm.2018.6013
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发表时间：
2018-06
影响因子：
2.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Wang Y
探讨转录因子同源盒基因（HOX基因）与小儿先天性马蹄内翻足（CCF）的关系。CCF组35例，对照组34例，均为无先天畸形儿。采用诱导型一氧化氮合酶（iNOS）和一氧化氮（NO）试剂盒检测对照组和CCF组血清中iNOS和NO水平。采用逆转录-聚合酶链反应（RT-PCR）检测两组组织中与炎症相关的白细胞介素-1 β（IL-1β）、IL-6和肿瘤坏死因子-α（TNF-α）。Western blot检测与细胞凋亡相关的脂肪酸合成酶（Fas）、Fas配体（FasL）和Bcl-2相关的X（Bax）以及HOX mRNA的表达，并对HOX在对照组和CCF组中的差异表达进行统计学分析。试剂盒检测结果显示，CCF组iNOS和NO的表达明显高于对照组，提示CCF组发生了严重的氧化损伤。RT-PCR检测炎症因子和细胞凋亡的结果显示，CCF组IL-1β、IL-6、TNF-α、Fas、FasL和Bax mRNA的表达明显高于对照组，提示CCF的发病机制与炎症和细胞凋亡有关。RT-PCR和western blot分析显示，HOX在CCF组织中呈高表达，且表达量明显高于对照组。方差分析结果显示，HOX在正常组织和CCF组织中的表达差异有统计学意义（P<0.01）。HOX的异常表达与CCF的发生发展密切相关，提示HOX在CCF中具有重要的研究价值，其作用机制与氧化损伤、炎症和凋亡有关。因此，HOX的表达显示出作为CCF诊断和治疗的指标的希望。
The relationship between transcription factor homeobox gene (HOX gene) and pediatric congenital clubfoot (CCF) was studied. The CCF group comprised 35 cases of children, and the control group compised 34 cases of children without congenital malformation. The levels of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in the serum of the control and CCF groups were measured using iNOS and NO kits. Interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) related to inflammation in the tissues of both groups were detected by reverse transcription-polymerase chain reaction (RT-PCR). Fatty acid synthase (Fas), Fas ligand (FasL) and Bcl-2-associated X (Bax) related to apoptosis as well as the expression of HOX mRNA, the expression of HOX in the control and CCF groups was detected by western blot analysis, and the differential expression of HOX in the control and CCF groups was statistically analyzed. Results of the kit detection showed that the expression of iNOS and NO in the CCF group were significantly higher than those in the control group, indicating that severe oxidative damage occurred in the CCF group. The results of detecting inflammatory factors and apoptosis by RT-PCR showed that the expression of IL-1β, IL-6, TNF-α, Fas, FasL and Bax mRNA in the CCF group was significantly higher than that in the control group, indicating pathogenesis of CCF was related to inflammation and apoptosis. RT-PCR and western blot analysis revealed HOX was highly expressed in the tissues of CCF, and the expression quantity was significantly stronger than that in the control group. The result of analysis of variance showed that the expression differences of HOX in normal and CCF tissues were statistically significant (P<0.01). Abnormal expression of HOX was closely related to the occurrence and development of CCF, indicating that HOX has important research value in CCF and this functional mechanism is related to oxidative damage, inflammation and apoptosis. Expression of HOX therefore shows promise as an indicator of CCF diagnosis and treatment.
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影响因子：
2.7
作者：
Ponseti, IV
通讯作者：
Ponseti, IV
DOI：
10.1073/pnas.95.22.13062
发表时间：
1998-10-27
影响因子：
11.1
作者：
Arenas-Mena, C;Martinez, P;Davidson, EH
通讯作者：
Davidson, EH
影响因子：
2.7
作者：
BEEMAN, RW;STUART, JJ;DENELL, RE
通讯作者：
DENELL, RE
DOI：
10.2106/00004623-196345020-00004
发表时间：
1963-01-01
影响因子：
5.3
作者：
PONSETI, IV;SMOLEY, EN
通讯作者：
SMOLEY, EN
DOI：
10.2106/00004623-195133030-00004
发表时间：
1951-01-01
影响因子：
5.3
作者：
STEWART, SF
通讯作者：
STEWART, SF