Effect of cinnamon water extract on monocyte-to-macrophage differentiation and scavenger receptor activity.
Effect of cinnamon water extract on monocyte-to-macrophage differentiation and scavenger receptor activity.
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DOI：
10.1186/1472-6882-14-90
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发表时间：
2014-03-07
影响因子：
--
通讯作者：
Kim YJ
中科院分区：
文献类型：
--
作者：
Kang H;Park SH;Yun JM;Nam TG;Kim YE;Kim DO;Kim YJ
水溶性肉桂提取物已被证明可以增加胰岛素敏感性并调节巨噬细胞活化，这是治疗肥胖或动脉粥样硬化的理想特征。我们目前的研究调查肉桂水提取物（CWE）是否可能影响单核细胞分化为巨噬细胞和巨噬细胞清道夫受体的活性，通常观察到动脉粥样硬化病变。我们研究了CWE对佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）刺激的THP-1细胞中各种表面标志物的表达和乙酰化低密度脂蛋白（LDL）的摄取的影响。分析PMA或巨噬细胞集落刺激因子（M-CSF）刺激的1型巨噬细胞清道夫受体（SRA）的蛋白水平。最后，细胞外信号相关激酶（ERK）1/2在SRA合成中的作用以及CWE对PMA刺激的ERK 1/2的影响被确定。CWE通过降低CD 11b、CD 36和SRA的表达及乙酰化LDL的摄取来抑制单核细胞的分化。CWE抑制M-CSF对SRA的上调，并调节PMA诱导的SRA合成所需的ERK 1/2活性。我们的研究结果表明，CWE能够干扰单核细胞分化和巨噬细胞清除活性，表明其在预防动脉粥样硬化病变的发展的潜力。
Water soluble cinnamon extract has been shown to increase insulin sensitivity and modulate macrophage activation, a desirable trait for the management of obesity or atherosclerosis. Our present study investigated whether cinnamon water extract (CWE) may influence the differentiation of monocytes into macrophages and the activity of macrophage scavenger receptors, commonly observed in atherosclerotic lesions. We investigated the effect of CWE on the expression of various surface markers and the uptake of acetylated low density lipoprotein (LDL) in phorbol-12-myristate-13-acetate (PMA)-stimulated THP-1 cells. The protein levels of PMA or macrophage-colony stimulating factor (M-CSF)-stimulated type 1 macrophage scavenger receptor (SRA) were analyzed. Finally, the role of extracellar signal-related kinase (ERK) 1/2 in SRA synthesis and the effect of CWE on PMA-stimulated ERK1/2 were determined. CWE inhibited the differentiation of monocyte by decreasing the expression of CD11b, CD36 and SRA and the uptake of acetyl LDL. CWE suppressed the upregulation of SRA by M-CSF and modulated ERK1/2 activity, which was required for PMA-induced SRA synthesis. Our results demonstrate that CWE was able to interfere with monocyte differentiation and macrophage scavenger activity, indicating its potential in preventing the development of atherosclerotic lesions.
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DOI：
10.1161/01.atv.14.5.798
发表时间：
1994-05-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
GENG, YJ;KODAMA, T;HANSSON, GK
通讯作者：
HANSSON, GK
影响因子：
4.7
作者：
Lu, Jianming;Zhang, Keqiang;Wen, Wei
通讯作者：
Wen, Wei
影响因子：
--
作者：
Hong, Joung-Woo;Yang, Ga-Eun;Kang, Hee
通讯作者：
Kang, Hee
DOI：
10.1084/jem.180.2.705
发表时间：
1994-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
de Villiers WJ;Fraser IP;Hughes DA;Doyle AG;Gordon S
通讯作者：
Gordon S
影响因子：
9.7
作者：
Kwon, Ho-Keun;Jeon, Won Kyung;Im, Sin-Hyeog
通讯作者：
Im, Sin-Hyeog