Deletion analysis of the SUP35 gene of the yeast Saccharomyces cerevisiae reveals two non‐overlapping functional regions in the encoded protein
Deletion analysis of the SUP35 gene of the yeast Saccharomyces cerevisiae reveals two non‐overlapping functional regions in the encoded protein
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对酿酒酵母 SUP35 基因的删除分析揭示了编码蛋白中两个不重叠的功能区
作者：
M. Ter‐Avanesyan;V. Kushnirov;A. Dagkesamanskaya;S. Didichenko;Y. Chernoff;S. Inge;V. Smirnov
SUP 35是酿酒酵母的全能抑制基因，编码由类似于延伸因子EF-1α的C末端部分和独特的253个氨基酸的N末端序列组成的蛋白质。SUP 35基因的12个截短版本通过缺失编码序列内部的片段而产生。这些缺失突变体的功能研究表明：（i）只有Sup 35蛋白的EF-1α样C末端部分对细胞活力是必需的;（ii）Sup 35蛋白的N末端部分或全长Sup 35蛋白的过表达降低了翻译保真度，导致全能抑制和[psi+]菌株生长减少;（iii）Sup 35蛋白的C末端部分的表达产生抗抑制表型;和（iv）Sup 35蛋白的N末端或C末端区段都可以结合80 S核糖体。因此，获得的数据定义了Sup 35蛋白内负责不同功能的两个结构域。
SUP35is an omnipotent suppressor gene of Saccharomyces cerevisiae coding for a protein consisting of a C‐terminal part similar to the elongation factor EF‐1α and a unique N‐terminal sequence of 253 amino acids. Twelve truncated versions of the SUP35 gene were generated by the deletion of fragments internal to the coding sequence. Functional studies of these deletion mutants showed that: (i) only the EF‐1α‐like C‐terminal part of the Sup35 protein is essential for the cell viability; (ii) overexpression of either the N‐terminal part of the Sup35 protein or the full‐length Sup35 protein decreases translational fidelity, resulting in omnipotent suppression and reduced growth of [psi+] strains; (iii) expression of the C‐terminal part of the Sup35 protein generates an antisuppressor phenotype; and (iv) both the N‐ or C‐terminal segments of the Sup35 protein can bind to 80S ribosomes. Thus, the data obtained define two domains within the Sup35 protein which are responsible for different functions.
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影响因子：
3.3
作者：
Cummins,CM;Gaber,RF;Culbertson,MR;Mann,R;Fink,GR
通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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影响因子：
3.3
作者：
Culbertson,MR;Gaber,RF;Cummins,CM
通讯作者：
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