Impact of pertussis-specific IgA, IgM, and IgG antibodies in mother's own breast milk and donor breast milk during preterm infant digestion.
Impact of pertussis-specific IgA, IgM, and IgG antibodies in mother's own breast milk and donor breast milk during preterm infant digestion.
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DOI：
10.1038/s41390-020-1031-2
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发表时间：
2021-04
影响因子：
3.6
通讯作者：
Dallas DC
中科院分区：
文献类型：
--
作者：
Demers-Mathieu V;Huston RK;Markell AM;McCulley EA;Martin RL;Dallas DC
本文研究了在早产儿消化过程中，来自母乳（MBM）和母乳（DBM）的百日咳毒素（PT）和丝状血凝素（FHA）特异性抗体同种型的存活情况。在产后8-9天和21-22天从20对早产母婴中采集饲料、胃和粪便样品。通过ELISA分析样本的抗FHA或抗PT免疫球蛋白A（伊加）、IgM和IgG。产后8-9 d，MBM组抗PT伊加、抗FHA IgG、抗PT IgG均低于DBM组，而抗FHA IgM均高于DBM组。DBM中的抗PT伊加、抗PT IgG和抗FHA IgG在产后两个时间的胃内容物中均减少，但MBM中的这些抗体在胃消化过程中保持稳定或增加。在8-9天时，食用MBM的婴儿胃内容物中抗FHA特异性伊加和IgM高于食用DBM的婴儿。所有百日咳抗体检测婴儿粪便在产后时间。在婴儿消化过程中，MBM的百日咳特异性抗体是稳定的，而DBM的抗百日咳伊加和IgG在胃内容物中减少。抗体的恒定区和可变区以及母体免疫似乎是其对蛋白水解消化和巴氏灭菌的稳定性的关键因素。
The survival of antibody isotypes specific to pertussis toxin (PT) and filamentous hemagglutinin (FHA) from mother’s own milk (MBM) and donor breast milk (DBM) during preterm infant digestion was investigated. Feed, gastric and stool samples were collected from 20 preterm mother-infant pairs at 8–9 days and 21–22 days postpartum. Samples were analyzed via ELISA for anti-FHA or anti-PT immunoglobulin A (IgA), IgM and IgG. Anti-PT IgA, anti-FHA IgG and anti-PT IgG were lower in MBM than DBM at 8–9 days postpartum, whereas anti-FHA IgM was higher in MBM than DBM. Anti-PT IgA, anti-PT IgG and anti-FHA IgG in DBM decreased in gastric contents at both postpartum times but those antibodies in MBM were stable or increased during gastric digestion. Anti-FHA specific IgA and IgM were higher in gastric contents from infants fed MBM than that fed DBM at 8–9 days. All pertussis antibodies were detected in infant stools at both postpartum times. Pertussis-specific antibodies from MBM were stable during infant digestion, whereas anti-pertussis IgA and IgG from DBM decreased in gastric contents. The constant region and variable region of antibodies and maternal immunization appear to be the critical factors for their stability to proteolytic digestion and pasteurization.
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DOI：
10.1001/archpedi.1952.02050040050006
发表时间：
1952-01-01
期刊：
AMA AMERICAN JOURNAL OF DISEASES OF CHILDREN
影响因子：
--
作者：
DECARLO, J;TRAMER, A;STARTZMAN, HH
通讯作者：
STARTZMAN, HH
DOI：
10.1097/mpg.0000000000001719
发表时间：
2018-02-01
影响因子：
2.9
作者：
Demers-Mathieu, Veronique;Nielsen, Soren Drud;Dallas, David C.
通讯作者：
Dallas, David C.
影响因子：
5.9
作者：
Demers-Mathieu, Veronique;Huston, Robert K.;Dallas, David C.
通讯作者：
Dallas, David C.
影响因子：
11.8
作者：
Halperin, B. A.;Morris, A.;Halperin, S. A.
通讯作者：
Halperin, S. A.
影响因子：
3.6
作者：
Sagrera, X;Ginovart, G;Vila, C
通讯作者：
Vila, C