Protective effect of Zhuyeqing liquor, a Chinese traditional health liquor, on acute alcohol-induced liver injury in mice.
Protective effect of Zhuyeqing liquor, a Chinese traditional health liquor, on acute alcohol-induced liver injury in mice.
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中国传统健康酒朱耶克酒对小鼠急性酒精引起的肝损伤的保护作用。
DOI：
10.1186/1476-9255-10-30
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发表时间：
2013-10-03
期刊：
影响因子：
--
通讯作者：
Wang JH
中科院分区：
文献类型：
--
作者：
Gao HY;Huang J;Wang HY;Du XW;Cheng SM;Han Y;Wang LF;Li GY;Wang JH
本研究首次评价了竹叶清酒对小鼠急性酒精性肝损伤的保护作用。除正常组小鼠外，其余各组动物均于灌胃ZYQL后每天4h灌胃给予50%乙醇12g/kg，连续14d。以血清天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)及肝组织还原型谷胱甘肽(GSH)、丙二醛(MDA)、超氧化物歧化酶(SOD)等生化指标评价其保护作用。免疫组织化学方法检测小鼠肝脏肿瘤坏死因子-α的表达。采用高效液相色谱联用法(HPLC-PDA)进行植物化学分析，并用UPLC-TOF-MS对各化合物的植物来源进行确证。结果表明，ZYQL可剂量依赖性地逆转脑损伤后的生化指标和组织病理学改变，具有明显的保护作用。高效液相色谱分析表明，ZYQL中含有黄酮类、环烯醚萜类、萜类和酚酸类化合物，可能是其活性成分。本研究论证了滋阴清肝灵的保肝作用，为其保健功能提供了科学依据。
The study first evaluated the hepatoprotective effect of Zhuyeqing Liquor (ZYQL) against acute alcohol-induced liver injury in mice. Animals were administered orally with 50% alcohol 12 ml/kg at 4 h after the doses of ZYQL everyday for fourteen consecutive days except mice in normal group. The protective effect was evaluated by biochemical parameters including serum aspartate transaminase (AST), alanine transferase (ALT), total-bilirubin (TBIL) and reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) in liver tissue. The result were confirmed histopathologically and the expression of TNF-α in mice liver was determined by immunohistochemistry analysis. HPLC-PDA was used for phytochemical analysis of ZYQL, and the plant source of each compound was claritied by UPLC-TOF-MS. The result showed that pretreatment with ZYQL exhibited a significant protective effect by reversing the biochemical parameters and histopathological changes in a dose depended manner. HPLC analysis indicated that ZYQL contained flavonoids, iridoids, terpenoids and phenolic acids, which might be the active chemicals. This study demonstrated the hepatoprotective activity of ZYQL, thus scientifically supported the function of its health care.
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