Characterization of Chitosan-Hyaluronic Acid Blended Membranes and Their Effects on the Growth of Keratocytes
Characterization of Chitosan-Hyaluronic Acid Blended Membranes and Their Effects on the Growth of Keratocytes
复制标题
壳聚糖-透明质酸混合膜的表征及其对角膜细胞生长的影响
DOI：
10.1177/096739111101900707
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发表时间：
2011-09
影响因子：
2.1
通讯作者：
Wu, Hai-Ge
中科院分区：
文献类型：
--
作者：
Yao, Zi-Ang;Wu, Hai-Ge
In order to construct a suitable scaffold for corneal cell culture and transplantation in vitro, various chitosan-hyaluronic acid blended membranes were prepared, and the properties of the blended membranes were studied. Rabbit keratocytes were seeded onto the blended membrane surfaces and the effects of the blended membranes on cell attachment and metabolism were investigated. The results showed that chitosan and hyaluronic acid (HA) were compatible in blended membranes. HA improved the homogeneity, crystallization and transparency, and decreased the tensile strength and hydrophilicity of blended membranes within the blending ratio of 1:0.05. Under the blending ratio of 1:0.02, HA reduced the harm that the chitosan membrane caused the cells and improved the biocompatibility between cells and membranes. Rabbit keratocytes grew and formed a confluent monolayer on the chitosan-hyaluronic acid blended membrane within a blending ratio of 1:0.02. All the results indicated that chitosan and HA were compatible within the blending ratio of 1:0.02, and the blended membranes had homogeneous structure, suitable physicochemical properties and biocompatibility with cells, this implied that the blended membranes could be used as a scaffold for corneal cell culture in vitro and as a potential carrier for corneal endothelial cell transplantation.
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影响因子：
14
作者：
Chen, Yen-Lin;Lee, Hsiao-Ping;Hu, Yu-Chen
通讯作者：
Hu, Yu-Chen
影响因子：
3.4
作者：
D. Thacharodi;K. Rao
通讯作者：
D. Thacharodi;K. Rao
DOI：
10.1007/s10856-008-3508-0
发表时间：
2008-12-01
影响因子：
3.7
作者：
Gao, Xingshuang;Liu, Wanshun;Yang, Chaozhong
通讯作者：
Yang, Chaozhong
DOI：
--
发表时间：
2006-08
期刊：
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
影响因子：
--
作者：
Zi-Ang Yao;Haige Wu;B. Han;Wanshun Liu
通讯作者：
Zi-Ang Yao;Haige Wu;B. Han;Wanshun Liu
影响因子：
14
作者：
Yoon‐Jeong Park;Yong-Moo Lee;Si Nae Park;Seung Yoon Sheen;C. Chung;Seung Jin Lee
通讯作者：
Yoon‐Jeong Park;Yong-Moo Lee;Si Nae Park;Seung Yoon Sheen;C. Chung;Seung Jin Lee