Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis.
Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis.
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DOI：
10.1093/jxb/ert040
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Yang S
中科院分区：
文献类型：
--
作者：
Guo L;Yang H;Zhang X;Yang S
已经报道了几种脂转移蛋白来调节植物对生物胁迫的反应；然而，脂转移蛋白是否也参与了非生物胁迫仍然是未知的。这项研究描述了脂转移蛋白LTP3在冰冻和干旱胁迫中的功能。LTP3蛋白广泛表达，定位于细胞质。一项生化研究表明，LTP3能够与脂质结合。过表达LTP3可在不影响CBF及其靶COR基因表达的情况下提高植物的耐寒性。进一步的分析表明，MYB96通过与LTP3启动子的直接结合来正向调控LTP3；持续地，高表达MYB96的转基因植株表现出更强的耐寒性。本研究还发现，功能缺失突变体ltp3对干旱胁迫敏感，而过表达植株则耐旱，其表型与myb96突变植株和MYB96过表达植株相似。综上所述，这些结果表明LTP3作为MYB96的一个靶标参与了植物对低温和干旱胁迫的耐受性。
Several lipid-transfer proteins were reported to modulate the plant response to biotic stress; however, whether lipid-transfer proteins are also involved in abiotic stress remains unknown. This study characterized the function of a lipid-transfer protein, LTP3, during freezing and drought stress. LTP3 was expressed ubiquitously and the LTP3 protein was localized to the cytoplasm. A biochemical study showed that LTP3 was able to bind to lipids. Overexpression of LTP3 resulted in constitutively enhanced freezing tolerance without affecting the expression of CBFs and their target COR genes. Further analyses showed that LTP3 was positively regulated by MYB96 via the direct binding to the LTP3 promoter; consistently, transgenic plants overexpressing MYB96 exhibited enhanced freezing tolerance. This study also found that the loss-of-function mutant ltp3 was sensitive to drought stress, whereas overexpressing plants were drought tolerant, phenotypes reminiscent of myb96 mutant plants and MYB96-overexpressing plants. Taken together, these results demonstrate that LTP3 acts as a target of MYB96 to be involved in plant tolerance to freezing and drought stress.
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影响因子：
5.3
作者：
Dong, Chun-Hai;Hu, Xiangyang;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
3.3
作者：
Buhot, N;Gomès, E;Blein, JP
通讯作者：
Blein, JP
影响因子：
0.6
作者：
DODGE, AD
通讯作者：
DODGE, AD
DOI：
10.1073/pnas.93.23.13404
发表时间：
1996-11-12
影响因子：
11.1
作者：
Artus, NN;Uemura, M;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF