Novel Liposomes for Efficient Transfection of β-Galactosidase Gene into COS-1 Cells
Novel Liposomes for Efficient Transfection of β-Galactosidase Gene into COS-1 Cells
复制标题
用于将 β-半乳糖苷酶基因有效转染至 COS-1 细胞的新型脂质体
DOI：
10.3164/jcbn.7.185
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发表时间：
1989
影响因子：
2.4
通讯作者：
K. Yagi
中科院分区：
文献类型：
--
作者：
T. Koshizaka;Y. Hayashi;K. Yagi
A pCH 110 plasmid encoding the β-galactosidase gene of E. coli was effectively entrapped in novel positively charged liposomes consisting of N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine (1:2:2, molar ratio) by an improved reverse-phase evaporation method. The DNA entrapping efficiency of these liposomes was 5-70 fold higher than that of liposomes containing no positively charged lipid. By using such positively charged liposomes, we could achieve transfection of the gene into COS-1 cells by simply adding the liposomes to the medium covering the cells; and the transfection efficiency exceeded that obtained by the calcium phosphate precipitation method, especially when a small amount of DNA was used.
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大肠杆菌 lacZ 基因融合在哺乳动物细胞中的表达和调控。
DOI：
--
发表时间：
1983
期刊：
Journal of molecular and applied genetics
影响因子：
--
作者：
Hall,CV;Jacob,PE;Ringold,GM;Lee,F
通讯作者：
Lee,F
DOI：
10.1016/s0021-9258(19)70482-7
发表时间：
1980-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Fraley;S. Subramani;P. Berg;D. Papahadjopoulos
通讯作者：
R. Fraley;S. Subramani;P. Berg;D. Papahadjopoulos
影响因子：
2.9
作者：
FRALEY, R;STRAUBINGER, RM;PAPAHADJOPOULOS, D
通讯作者：
PAPAHADJOPOULOS, D
DOI：
10.1073/pnas.84.21.7413
发表时间：
1987-11-01
影响因子：
11.1
作者：
FELGNER, PL;GADEK, TR;DANIELSEN, M
通讯作者：
DANIELSEN, M