Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in PC12 cells and neurite outgrowth in zebrafish embryos.
Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in PC12 cells and neurite outgrowth in zebrafish embryos.
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DOI：
10.1371/journal.pone.0026461
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Huang CJ
中科院分区：
文献类型：
--
作者：
Huang KY;Chen GD;Cheng CH;Liao KY;Hung CC;Chang GD;Hwang PP;Lin SY;Tsai MC;Khoo KH;Lee MT;Huang CJ
M6A是哺乳动物神经元中表达的蛋白脂质蛋白（PLP/DM20）家族成员，首次用单克隆抗体表达克隆分离得到。M6A过表达可诱导神经细胞形成丝状伪足；然而，其潜在的机制在很大程度上是未知的。可能是由于基因复制，在斑马鱼基因组中有两个M6A的类似物，M6Aa和M6Ab。在本研究中，我们以斑马鱼为模型系统，研究了斑马鱼M6Ab在PC12细胞丝足形成和斑马鱼胚胎神经突生长中的作用。我们证明了斑马鱼M6Ab促进ngf处理的PC12细胞中广泛的丝足形成，这与哺乳动物M6A的功能相似。斑马鱼M6Ab丝氨酸263的磷酸化有助于这种诱导。转染S263A突变蛋白大大减少了PC12细胞中丝足的形成。在斑马鱼胚胎中，只有S263D能诱导神经突生长。我们的研究结果表明，斑马鱼M6Ab丝氨酸263的磷酸化状态对其在调节丝足形成和神经突生长中的作用至关重要。
Mammalian M6A, a member of the proteolipid protein (PLP/DM20) family expressed in neurons, was first isolated by expression cloning with a monoclonal antibody. Overexpression of M6A was shown to induce filopodium formation in neuronal cells; however, the underlying mechanism of is largely unknown. Possibly due to gene duplication, there are two M6A paralogs, M6Aa and M6Ab, in the zebrafish genome. In the present study, we used the zebrafish as a model system to investigate the role of zebrafish M6Ab in filopodium formation in PC12 cells and neurite outgrowth in zebrafish embryos. We demonstrated that zebrafish M6Ab promoted extensive filopodium formation in NGF-treated PC12 cells, which is similar to the function of mammalian M6A. Phosphorylation at serine 263 of zebrafish M6Ab contributed to this induction. Transfection of the S263A mutant protein greatly reduced filopodium formation in PC12 cells. In zebrafish embryos, only S263D could induce neurite outgrowth. Our results reveal that the phosphorylation status of zebrafish M6Ab at serine 263 is critical for its role in regulating filopodium formation and neurite outgrowth.
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影响因子：
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作者：
Amores, A;Suzuki, T;Postlethwait, JH
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.84.16.5665
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10.1016/j.bbrc.2004.06.172
发表时间：
2004-08-20
影响因子：
3.1
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通讯作者：
Chou, CM