Quantitative and molecular analyses of radiation-induced mutation in AS52 cells.
Quantitative and molecular analyses of radiation-induced mutation in AS52 cells.
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AS52 细胞辐射诱导突变的定量和分子分析。
DOI：
10.2307/3576723
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发表时间：
1986
影响因子：
3.4
通讯作者：
A. Hsie
中科院分区：
文献类型：
--
作者：
L. Stankowski;A. Hsie
利用psv2gpt转化和野生型中国仓鼠卵巢（CHO）细胞系在分子水平上研究辐射诱导突变。这个被命名为AS52的转化体是由一个缺乏次黄嘌呤-鸟嘌呤磷酸化核糖转移酶（HPRT）的CHO细胞系构建而成的，它含有一个大肠杆菌黄嘌呤-鸟嘌呤磷酸化核糖转移酶（XPRT）基因（gpt）的单一功能拷贝，该基因稳定地整合到中国仓鼠基因组中。AS52和野生型CHO-K1-BH4细胞对紫外光和X射线表现出相似的细胞毒性反应；然而，GPT和HPRT基因座的突变诱导存在显著差异。利用Southern-blot杂交技术对辐照后产生的HPRT和XPRT突变体进行了分析。大多数XPRT（21/26）和所有HPRT（23/23）突变体在紫外光诱导下表现出与其亲本细胞系难以区分的杂交模式。相比之下，X照射诱导的所有XPRT（26/26）和大多数HPRT突变体（15/21）分别含有影响部分或全部gpt和HPRT位点的缺失突变。这些结果表明，X射线主要诱导缺失突变，而紫外线可能诱导两个位点的点突变。
pSV2gpt-Transformed and wild-type Chinese hamster ovary (CHO) cell lines have been used to study radiation-induced mutation at the molecular level. The transformant, designated AS52, was constructed from a hypoxanthine-guanine phosphoribosyl transferase (HPRT)-deficient CHO cell line and contains a single, functional copy of the Escherichia coli xanthine-guanine phosphoribosyl transferase (XPRT) gene (gpt) stably integrated into the Chinese hamster genome. AS52 and wild-type CHO-K1-BH4 cells exhibit similar cytotoxic responses to uv light and X rays; however, significant differences occur in mutation induction at the gpt and hprt loci. A number of HPRT and XPRT mutants which arose following irradiation were analyzed by Southern-blot hybridization. Most XPRT (21/26) and all HPRT (23/23) mutants induced by uv light exhibited hybridization patterns indistinguishable from their parental cell lines. In contrast, all XPRT (26/26) and most HPRT mutants (15/21) induced by X irradiation contained deletion mutations affecting some or all of the gpt and hprt loci, respectively. These results indicate that X rays induce predominantly deletion mutations, while uv light is likely to induce point mutations at both loci.
电离辐射产生的 DNA 碱基损伤的检测和修复。
DOI：
10.1002/em.2860050514
发表时间：
1983
期刊：
Environmental mutagenesis
影响因子：
--
作者：
Wallace,SS
通讯作者：
Wallace,SS
小鼠和中国仓鼠的次黄嘌呤鸟嘌呤磷酸核糖基转移酶基因：cDNA重组体的构建和序列分析。
影响因子：
14.9
作者：
Konecki,DS;Brennand,J;Fuscoe,JC;Caskey,CT;Chinault,AC
通讯作者：
Chinault,AC