Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified.
Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified.
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FLT3配体处理的小鼠功能成熟的树突状细胞数量的急剧增加：确定的多个树突状细胞亚群。
DOI：
10.1084/jem.184.5.1953
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发表时间：
1996-11-01
影响因子：
15.3
通讯作者：
McKenna, HJ
中科院分区：
文献类型：
--
作者：
Maraskovsky, E;Brasel, K;Teepe, M;Roux, ER;Lyman, SD;Shortman, K;McKenna, HJ
树突状细胞（DC）是T细胞最有效的APC。 DC作为抗肿瘤和传染病免疫疗法的临床用途受到正常组织和终末分化状态的痕量水平和可及性的限制。在本研究中，每天注射人类FLT3配体（FLT3L）在小鼠中导致共表达特征DC标记II MHC，CD11C，DEC205和CD86的细胞的数值急剧增加。相比之下，在用GM-CSF，GM-CSF Plus IL-4，C-KIT配体（C-KITL）或G-CSF治疗的小鼠中，II+ CD11C+细胞没有显着增加。使用CD8 alpha和CD11b表达，在FLT3L处理的小鼠的脾脏中鉴定了五个不同的DC亚群。这些细胞表现出遮盖的和树突状的过程，并且与稀有的稀有DC一样有效，从未处理的小鼠的脾脏中分离出来，在向T细胞中呈现异轴或可溶性Ag，或在体内启动Ag特异性T细胞反应。在骨髓，胃 - 肠淋巴组织（GALT），肝脏，淋巴结，肺，周围血液，腹膜腔，脾脏，脾和胸腺中检测到DC的急剧数值增加。这些结果表明，FLT3L可用于扩大体内功能成熟的DC的数量，用于临床免疫疗法。
Dendritic cells (DC) are the most efficient APC for T cells. The clinical use of DC as vectors for anti-tumor and infectious disease immunotherapy has been limited by their trace levels and accessibility in normal tissue and terminal state of differentiation. In the present study, daily injection of human Flt3 ligand (Flt3L) into mice results in a dramatic numerical increase in cells co-expressing the characteristic DC markers-class II MHC, CD11c, DEC205, and CD86. In contrast, in mice treated with either GM-CSF, GM-CSF plus IL-4, c-kit ligand (c-kitL), or G-CSF, class II+ CD11c+ cells were not significantly increased. Five distinct DC subpopulations were identified in the spleen of Flt3L-treated mice using CD8 alpha and CD11b expression. These cells exhibited veiled and dendritic processes and were as efficient as rare, mature DC isolated from the spleens of untreated mice at presenting allo-Ag or soluble Ag to T cells, or in priming an Ag-specific T cell response in vivo. Dramatic numerical increases in DC were detected in the bone marrow, gastro-intestinal lymphoid tissue (GALT), liver, lymph nodes, lung, peripheral blood, peritoneal cavity, spleen, and thymus. These results suggest that Flt3L could be used to expand the numbers of functionally mature DC in vivo for use in clinical immunotherapy.
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影响因子：
4.4
作者：
Sallusto, Federica;Lanzavecchia, Antonio
通讯作者：
Lanzavecchia, Antonio
DOI：
10.1073/pnas.90.7.3038
发表时间：
1993-04-01
影响因子：
11.1
作者：
INABA, K;INABA, M;STEINMAN, RM
通讯作者：
STEINMAN, RM
影响因子：
64.8
作者：
ARDAVIN, C;WU, L;SHORTMAN, K
通讯作者：
SHORTMAN, K
影响因子：
20.3
作者：
Brasel, K;McKenna, HJ;Lyman, SD
通讯作者：
Lyman, SD
影响因子：
64.5
作者：
LYMAN, SD;JAMES, L;BECKMANN, MP
通讯作者：
BECKMANN, MP