Polycaprolactone-coated 3D printed tricalcium phosphate scaffolds for bone tissue engineering: in vitro alendronate release behavior and local delivery effect on in vivo osteogenesis.
Polycaprolactone-coated 3D printed tricalcium phosphate scaffolds for bone tissue engineering: in vitro alendronate release behavior and local delivery effect on in vivo osteogenesis.
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DOI：
10.1021/am501048n
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发表时间：
2014-07-09
影响因子：
9.5
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
--
作者：
Tarafder, Solaiman;Bose, Susmita
关键词：
本工作的目的是评估通过聚己内酯（PCL）涂层的阿仑膦酸钠（AD）体外释放行为对使用PCL涂层的3D打印互连多孔磷酸三钙（TCP）支架体内骨形成的影响。在较低pH（5.0）下，AD和Ca ~（2+）离子释放量高于较高pH（7.4）。AD和Ca 2+释放，表面形态，和释放后的相分析表明，基质降解占主导地位的TCP溶解引起的AD释放。PCL涂层显示了其控制和持续释放AD的有效性。在Sprague-Dawley大鼠的远端股骨缺损中测试六种不同的支架组合物，即（i）TCP（裸TCP）、（ii）TCP + AD（AD涂覆的TCP）、（iii）TCP + PCL（PCL涂覆的TCP）、（iv）TCP + PCL + AD、（v）TCP + AD + PCL和（vi）TCP + AD + PCL + AD，持续6周和10周。从组织形态学上观察到支架的微孔和大孔内有良好的成骨作用。组织形态学分析显示6周后TCP + AD + PCL支架中新骨形成最多。PCL对TCP的生物活性和体内骨形成无不良影响。与裸TCP和仅用PCL涂覆的TCP相比，所有具有AD的支架显示出更高的骨形成和降低的TRAP（抗酒石酸酸性磷酸酶）阳性细胞活性。裸TCP支架显示出最高的TRAP阳性细胞活性，其次是TCP + PCL支架，而TCP + AD支架显示出最低的TRAP活性。6周后，与TCP + AD支架相比，在TCP + AD + PCL中观察到更高的TRAP阳性细胞活性。我们的研究结果表明，在体内局部AD从PCL涂层的3DP TCP支架传递可以进一步诱导增加早期骨形成。
The aim of this work was to evaluate the effect of in vitro alendronate (AD) release behavior through polycaprolactone (PCL) coating on in vivo bone formation using PCL-coated 3D printed interconnected porous tricalcium phosphate (TCP) scaffolds. Higher AD and Ca2+ ion release was observed at lower pH (5.0) than that at higher pH (7.4). AD and Ca2+ release, surface morphology, and phase analysis after release indicated a matrix degradation dominated AD release caused by TCP dissolution. PCL coating showed its effectiveness for controlled and sustained AD release. Six different scaffold compositions, namely, (i) TCP (bare TCP), (ii) TCP + AD (AD-coated TCP), (iii) TCP + PCL (PCL-coated TCP), (iv) TCP + PCL + AD, (v) TCP + AD + PCL, and (vi) TCP + AD + PCL + AD were tested in the distal femoral defect of Sprague–Dawley rats for 6 and 10 weeks. An excellent bone formation inside the micro and macro pores of the scaffolds was observed from histomorphology. Histomorphometric analysis revealed maximum new bone formation in TCP + AD + PCL scaffolds after 6 weeks. No adverse effect of PCL on bioactivity of TCP and in vivo bone formation was observed. All scaffolds with AD showed higher bone formation and reduced TRAP (tartrate resistant acid phosphatase) positive cells activity compared to bare TCP and TCP coated with only PCL. Bare TCP scaffolds showed the highest TRAP positive cells activity followed by TCP + PCL scaffolds, whereas TCP + AD scaffolds showed the lowest TRAP activity. A higher TRAP positive cells activity was observed in TCP + AD + PCL compared to TCP + AD scaffolds after 6 weeks. Our results show that in vivo local AD delivery from PCL-coated 3DP TCP scaffolds could further induce increased early bone formation.
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影响因子：
158.5
作者：
LIBERMAN, UA;WEISS, SR;KARPF, DB
通讯作者：
KARPF, DB
影响因子：
14
作者：
Kim, HW;Knowles, JC;Kim, HE
通讯作者：
Kim, HE
影响因子：
14
作者：
Levengood, Sheeny K. Lan;Polak, Samantha J.;Johnson, Amy J. Wagoner
通讯作者：
Johnson, Amy J. Wagoner
影响因子：
4.1
作者：
Bolgen, Nimet;Yang, Ying;Piskin, Erhan
通讯作者：
Piskin, Erhan
影响因子：
5.3
作者：
Garbuz, Donald S.;Hu, Youxin;Duncan, Clive P.
通讯作者：
Duncan, Clive P.