DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.
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DOI：
10.1038/35020000
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发表时间：
2000-08-03
期刊：
影响因子：
64.8
通讯作者：
Fraser CM
中科院分区：
文献类型：
--
作者：
Heidelberg JF;Eisen JA;Nelson WC;Clayton RA;Gwinn ML;Dodson RJ;Haft DH;Hickey EK;Peterson JD;Umayam L;Gill SR;Nelson KE;Read TD;Tettelin H;Richardson D;Ermolaeva MD;Vamathevan J;Bass S;Qin H;Dragoi I;Sellers P;McDonald L;Utterback T;Fleishmann RD;Nierman WC;White O;Salzberg SL;Smith HO;Colwell RR;Mekalanos JJ;Venter JC;Fraser CM
在这里，我们确定了革兰氏阴性，γ-变形杆菌埃尔托尔霍乱弧菌N16961的完整基因组序列为4,033,460个碱基对(Bp)。该基因组由2,961,146和1,072,314 bp的两条环形染色体组成，共编码3,885个开放阅读框。绝大多数可识别的基本细胞功能基因(如DNA复制、转录、翻译和细胞壁生物合成)和致病性基因(如毒素、表面抗原和粘附素)位于大染色体上。相比之下，与大染色体(42%)相比，小染色体包含更多的假设基因(59%)，而且还包含更多似乎来自γ-变形杆菌以外的基因。小染色体还携带一个基因捕获系统(整合子岛)和寄主‘成瘾’基因，这些基因通常存在于质粒上；因此，小染色体最初可能是一个由祖先弧菌物种捕获的巨质粒。霍乱弧菌基因组序列为理解自由生活的环境有机体是如何成为重要的人类细菌病原体提供了一个起点。本文的在线版本(DOI：35020000/10.1038)包含补充材料，可供授权用户使用。
Here we determine the complete genomic sequence of the Gram negative, γ-Proteobacterium Vibrio cholerae El Tor N16961 to be 4,033,460 base pairs (bp). The genome consists of two circular chromosomes of 2,961,146 bp and 1,072,314 bp that together encode 3,885 open reading frames. The vast majority of recognizable genes for essential cell functions (such as DNA replication, transcription, translation and cell-wall biosynthesis) and pathogenicity (for example, toxins, surface antigens and adhesins) are located on the large chromosome. In contrast, the small chromosome contains a larger fraction (59%) of hypothetical genes compared with the large chromosome (42%), and also contains many more genes that appear to have origins other than the γ-Proteobacteria. The small chromosome also carries a gene capture system (the integron island) and host ‘addiction’ genes that are typically found on plasmids; thus, the small chromosome may have originally been a megaplasmid that was captured by an ancestral Vibrio species. The V. cholerae genomic sequence provides a starting point for understanding how a free-living, environmental organism emerged to become a significant human bacterial pathogen. The online version of this article (doi:10.1038/35020000) contains supplementary material, which is available to authorized users.
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