Identification of an adipose tissue-resident pro-preadipocyte population.
Identification of an adipose tissue-resident pro-preadipocyte population.
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DOI：
10.1016/j.celrep.2023.112440
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发表时间：
2023-05-30
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
阐明干细胞分化过程中的过渡阶段，可以促进我们对生物学的理解，并促进对治疗机会的识别。然而，将祖细胞与其他细胞类型区分开来并将其置于上位性途径中是具有挑战性的。这在脂肪细胞谱系中得到了例证，来自脂肪组织的基质血管部分（SVF）富含祖细胞，但也含有异质细胞群。单细胞RNA测序（scRNA-seq）已经开始促进SVF中细胞类型的反褶积，并且分层结构正在出现。在这里，我们使用scRNA-seq来发现SVF中由特定表达谱区分的CD31 - CD45 -细胞群。此外，我们将这个群体置于先前定义的前脂肪细胞群体上游的上位性途径上。最后，我们发现了具有广泛意义的这一群体的功能特性，包括揭示调节脂肪形成的生理机制。Chen等人在白色脂肪组织中发现了一种独特的脂肪祖细胞群，它与前脂肪细胞在上位途径中处于上游。通过小鼠和人的原代脂肪细胞，他们发现Noct是对这些细胞（他们称之为前脂肪细胞）具有功能影响的决定性标记物。
Elucidating the transitional stages that define the pathway stem cells progress through during differentiation advances our understanding of biology and fosters the identification of therapeutic opportunities. However, distinguishing progenitor cells from other cell types and placing them in an epistatic pathway is challenging. This is exemplified in the adipocyte lineage, where the stromal vascular fraction (SVF) from adipose tissue is enriched for progenitor cells but also contains heterogeneous populations of cells. Single-cell RNA sequencing (scRNA-seq) has begun to facilitate the deconvolution of cell types in the SVF, and a hierarchical structure is emerging. Here, we use scRNA-seq to discover a population of CD31− CD45− cells in the SVF that are distinguished by a specific expression profile. Further, we place this population on an epistatic pathway upstream of the previously defined preadipocyte population. Finally, we discover functional properties of this population with broad implications, including revealing physiological mechanisms that regulate adipogenesis. Chen et al. identify a distinct population of adipose progenitor cells, which is upstream in an epistatic pathway with preadipocytes, in white adipose tissue. Using both primary mouse and human adipose cells, they reveal that Noct is a defining marker with functional effects on these cells, which they term pro-preadipocytes.
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影响因子：
64.8
作者：
通讯作者：
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影响因子：
29
作者：
Berry R;Jeffery E;Rodeheffer MS
通讯作者：
Rodeheffer MS
脂肪组织中的细胞异质性。
影响因子：
18.2
作者：
Corvera S
通讯作者：
Corvera S
影响因子：
16.6
作者：
Estrella, Michael A.;Du, Jin;Korennykh, Alexei
通讯作者：
Korennykh, Alexei
影响因子：
8.8
作者：
Park, Byung Ouk;Ahrends, Robert;Teruel, Mary N.
通讯作者：
Teruel, Mary N.