Oral administration of bovine milk from cows hyperimmunized with intestinal bacterin stimulates lamina propria T lymphocytes to produce Th1-biased cytokines in mice.
Oral administration of bovine milk from cows hyperimmunized with intestinal bacterin stimulates lamina propria T lymphocytes to produce Th1-biased cytokines in mice.
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口服给予肠道菌苗超免疫的奶牛的牛奶会刺激小鼠固有层 T 淋巴细胞产生 Th1 偏向的细胞因子。
DOI：
10.3390/ijms15045458
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发表时间：
2014-03-28
影响因子：
5.6
通讯作者：
Yoshikai Y
中科院分区：
文献类型：
--
作者：
Wang Y;Lin L;Yin C;Othtani S;Aoyama K;Lu C;Sun X;Yoshikai Y
本研究的目的是研究口服一种专有细菌（免疫乳“Sustaina”）高免疫奶牛的牛奶对成年小鼠肠道粘膜免疫的影响。给C57BL/6小鼠口服免疫乳或对照乳2周后，检测正常小鼠和葡聚糖硫酸钠（DSS）诱导结肠炎小鼠结肠固有层淋巴细胞数量和细胞因子产量。我们发现免疫乳喂养小鼠结肠固有层中IFN-γ和IL-10水平升高，而IL-17A和IL-4水平下降。有趣的是，口服免疫乳可部分改善DSS诱导的急性结肠炎。dss诱导结肠炎免疫乳喂养小鼠结肠固有层中TNF-α、IFN-γ水平升高，IL-6、IL-17A、IL-4水平降低。我们的研究结果表明，免疫乳可能刺激CD4+ T细胞向Th1型反应极化，而相反地抑制Th17和Th2细胞在小鼠大肠LP中的反应。结果提示，该免疫乳能促进肠道稳态的维持，增强对感染的保护作用，并能减轻小鼠急性结肠炎的症状。
The goal of this study was to examine the effects of oral administration of bovine milk from cows hyperimmunized with a proprietary bacterin (immune milk “Sustaina”) on mucosal immunity in the intestine of adult mice. C57BL/6 mice were orally given immune or control milk for two weeks, and then lymphocyte population and the cytokine production in lamina propria of colon in normal mice and mice induced colitis by dextran sulphate sodium (DSS) were detected. We found that the levels of IFN-γ and IL-10 increased, but the levels of IL-17A and IL-4, decreased in lamina propria of colon in immune milk-fed mice as compared with those in control milk-fed mice. Interestingly, oral administration of immune milk partially improved the acute colitis induced by DSS. The levels of TNF-α and IFN-γ increased, but IL-6, IL-17A and IL-4 decreased in lamina propria (LP) of colon in immune milk-fed mice with DSS-induced colitis. Our results suggest that immune milk may stimulate CD4+ T cells to polarize towards a Th1 type response, but contrarily suppress Th17 and Th2 cells responses in large intestinal LP of mice. The results indicate that this kind of immune milk has is able to promote the maintainance of intestinal homeostasis and enhance protection against infection, and could alleviate the symptoms of acute colitis in mice.
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DOI：
10.1126/science.1195568
发表时间：
2010-12-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lee YK;Mazmanian SK
通讯作者：
Mazmanian SK
DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
29.4
作者：
Fina, Daniele;Sarra, Massimiliano;Monteleone, Giovanni
通讯作者：
Monteleone, Giovanni
T细胞分化，成熟和功能中细胞因子信号传导（SOC）的抑制。
影响因子：
16.8
作者：
Palmer DC;Restifo NP
通讯作者：
Restifo NP
影响因子：
29.7
作者：
Kaser A;Zeissig S;Blumberg RS
通讯作者：
Blumberg RS