Comparative characterization of two intracellular Ca²⁺-release channels from the red flour beetle, Tribolium castaneum.
Comparative characterization of two intracellular Ca²⁺-release channels from the red flour beetle, Tribolium castaneum.
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赤拟谷盗两种细胞内 Ca2+ 释放通道的比较表征
DOI：
10.1038/srep06702
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发表时间：
2014-10-21
影响因子：
4.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Liu Y;Li C;Gao J;Wang W;Huang L;Guo X;Li B;Wang J
Ryanodine受体（RyRs）和1，4，5-三磷酸肌醇受体（IP 3Rs）是四聚体细胞内钙释放通道（CRCs）家族的成员。虽然众所周知，在哺乳动物中，RyRs和IP 3Rs调节多种生理过程，但这两种CRCs在昆虫发育和生理学中的作用仍然知之甚少。在这项研究中，我们克隆和功能特性的RyR和IP 3R cDNA（命名为TcRyR和TcIP 3R）从赤拟谷盗，赤拟谷盗。该复合TcRyR基因含有15，285 bp的ORF，编码5，094个氨基酸残基的蛋白质。TcIP 3R含有一个8，175 bp的ORF，编码2，724个氨基酸的蛋白质。TcRyR和TcIP 3R的表达分析显示，T.栗在不同的发展阶段。当TcRyR的转录水平被RNA干扰（RNAi）抑制时，观察到成虫后翅的异常折叠，而TcIP 3R的RNAi介导的敲低导致有缺陷的幼虫-蛹和蛹-成虫变态。这些结果表明，TcRyR是T.推测IP 3R介导的钙释放可能在昆虫蜕皮和变态过程中对蜕皮激素的合成和释放起重要调节作用。
Ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs) are members of a family of tetrameric intracellular Ca2+-release channels (CRCs). While it is well known in mammals that RyRs and IP3Rs modulate multiple physiological processes, the roles of these two CRCs in the development and physiology of insects remain poorly understood. In this study, we cloned and functionally characterized RyR and IP3R cDNAs (named TcRyR and TcIP3R) from the red flour beetle, Tribolium castaneum. The composite TcRyR gene contains an ORF of 15,285 bp encoding a protein of 5,094 amino acid residues. The TcIP3R contains an 8,175 bp ORF encoding a protein of 2,724 amino acids. Expression analysis of TcRyR and TcIP3R revealed significant differences in mRNA expression levels among T. castaneum during different developmental stages. When the transcript levels of TcRyR were suppressed by RNA interference (RNAi), an abnormal folding of the adult hind wings was observed, while the RNAi-mediated knockdown of TcIP3R resulted in defective larval–pupal and pupal–adult metamorphosis. These results suggested that TcRyR is required for muscle excitation-contraction (E-C) coupling in T. castaneum and that calcium release via IP3R might play an important role in regulating ecdysone synthesis and release during molting and metamorphosis in insects.
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