Urinary DNA adductomics - A novel approach for exposomics.
Urinary DNA adductomics - A novel approach for exposomics.
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DOI：
10.1016/j.envint.2018.10.041
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发表时间：
2018-12
影响因子：
11.8
通讯作者：
Chao MR
中科院分区：
文献类型：
--
作者：
Cooke MS;Hu CW;Chang YJ;Chao MR
暴露是一个概念，包括内部和外部环境暴露的整体，从概念开始。评估整个生命过程中的暴露是一项重大挑战，例如，可能根本不存在全面评估所有暴露的方法/技术，或者它们可能不适用于人群，或者可能灵敏度不足。细胞DNA内合组学旨在确定细胞基因组中DNA加合物的总数。然而，应用于人群需要对组织进行必要的侵入性采样，以获得足够的DNA进行敏感分析，这可能代表后勤和IRB挑战，特别是在调查脆弱人群时。为了避免这种情况，我们最近将DNA内合组学应用于尿液，检测了一系列预期和意外的2 ' -脱氧核糖核苷DNA加合物。然而，碱基切除修复，非大体积DNA加合物的主要DNA修复途径，以及自发去嘌呤等过程，产生核碱基加合物。在此，我们提出了一种同时评估2 ' -脱氧核糖核苷和核碱基加合物的策略，使用广泛使用的质谱平台（即三重四极杆串联质谱）。通过评估DNA加合物的总量，这将为非侵入性、生物监测环境暴露提供一种急需的DNA加合物方法；有助于评估整个生命过程中的暴露量。
The exposome is a concept that encompasses the totality of internal and external environmental exposures, from conception onwards. Evaluation of the exposome, across the lifecourse represents a significant challenge, e.g., methods/technology may simply not exist to comprehensively assess all exposures, or they may not be applicable to human populations, or may have insufficient sensitivity. Cellular DNA adductomics aims to determine the totality of DNA adducts in the cellular genome. However, application to human populations requires the necessarily invasive sampling of tissue, to obtain sufficient DNA for sensitive analysis, which can represent a logistical and IRB challenge, particularly when investigating vulnerable populations. To circumvent this, we recently applied DNA adductomics to urine, detecting a range of expected and unexpected 2’-deoxyribonucleoside DNA adducts. However, base excision repair, the main DNA repair pathway for non-bulky DNA adducts, and processes such as spontaneous depurination, generate nucleobase adducts. Herein we propose a strategy to simultaneously assess 2’-deoxyribonucleoside and nucleobase adducts, using a widely used mass spectrometic platform (i.e., triple quadrupole tandem mass spectrometry). This will provide a much needed DNA adductomic approach for non-invasively, biomonitoring environmental exposures, through assessing the totality of DNA adducts; contributing to the evaluation of the exposome, across the life-course.
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DOI：
10.1016/j.mrfmmm.2005.01.022
发表时间：
2005-07-01
影响因子：
2.3
作者：
Cooke, MS;Evans, MD;Olinski, R
通讯作者：
Olinski, R
影响因子：
4.8
作者：
Gedik, CM;Collins, A;Vaughan, N
通讯作者：
Vaughan, N
影响因子：
3.8
作者：
Hansen, Thomas V. O.;Simonsen, Mette K.;Hundrup, Yrsa Andersen
通讯作者：
Hundrup, Yrsa Andersen
影响因子：
3.9
作者：
Collins, AR;Cadet, J;Viña, J
通讯作者：
Viña, J
影响因子：
2.9
作者：
Jaruga, Pawel;Rozalski, Rafal;Dizdaroglu, Miral
通讯作者：
Dizdaroglu, Miral