RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers.
RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers.
复制标题
RNA结构和荧光灯适体的细胞应用。
DOI：
10.1002/anie.201806482
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发表时间：
2019-01-28
期刊：
影响因子：
--
通讯作者：
Hennig S
中科院分区：
文献类型：
--
作者：
Neubacher S;Hennig S
RNA的细胞功能不仅限于其作为蛋白质合成蓝图的作用。特别是非编码RNA，如snRNA，lncRNA，miRNA，发挥重要作用。随着越来越多的RNA被鉴定，众所周知，转录组的数量远远超过蛋白质组。这强调了功能RNA表征的重要性，以及进一步开发这些研究工具的必要性，其中许多研究仍处于起步阶段。荧光发光适体（FLAP）是可以结合无毒的、细胞可渗透的小分子荧光团并在结合后将其荧光增强许多数量级的RNA序列。FLAP可以使用标准分子生物学工具在DNA水平上编码，随后通过细胞机制转录成RNA，因此它们可以用作荧光RNA标签（FLAP-tags）。在这篇小综述中，我们简要概述了已经开发的荧光团及其结合RNA适体，特别关注已发表的晶体结构。总结了当前和未来的细胞FLAP应用，重点是使用分裂FLAP和Förster共振能量转移（FRET）系统研究RNA-RNA和RNA-蛋白质相互作用。
The cellular functions of RNA are not limited to their role as blueprints for protein synthesis. In particular, noncoding RNA, such as, snRNAs, lncRNAs, miRNAs, play important roles. With increasing numbers of RNAs being identified, it is well known that the transcriptome outnumbers the proteome by far. This emphasizes the great importance of functional RNA characterization and the need to further develop tools for these investigations, many of which are still in their infancy. Fluorescent light‐up aptamers (FLAPs) are RNA sequences that can bind nontoxic, cell‐permeable small‐molecule fluorogens and enhance their fluorescence over many orders of magnitude upon binding. FLAPs can be encoded on the DNA level using standard molecular biology tools and are subsequently transcribed into RNA by the cellular machinery, so that they can be used as fluorescent RNA tags (FLAP‐tags). In this Minireview, we give a brief overview of the fluorogens that have been developed and their binding RNA aptamers, with a special focus on published crystal structures. A summary of current and future cellular FLAP applications with an emphasis on the study of RNA–RNA and RNA–protein interactions using split‐FLAP and Förster resonance energy transfer (FRET) systems is given.
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