What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories.
What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories.
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DOI：
10.1007/s10096-010-1104-y
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发表时间：
2011-03
影响因子：
4.5
通讯作者：
von Koenig, C. H. Wirsing
中科院分区：
文献类型：
--
作者：
Guiso, N.;Berbers, G.;Fry, N. K.;He, Q.;Riffelmann, M.;von Koenig, C. H. Wirsing
百日咳杆菌特异性抗体可通过酶联免疫吸附试验（ELISA）或多重免疫试验检测。检测使用纯化或混合抗原，只有百日咳毒素（PT）对B具有特异性。百日咳。结果的解释可以基于双样本或单样本血清学，使用一个或两个临界值。EU Pertstrain小组建议：（i）ELISA和多重免疫测定法应使用纯化的非脱毒PT作为抗原，它们应具有宽的线性范围，并应以每毫升国际单位（IU/ml）定量表示结果;（ii）应使用50 - 120 IU/ml之间的IgG-抗PT单血清血清学的单一或双重诊断临界值，和诊断血清学不能有效地解释接种无细胞百日咳（aP）疫苗后一年;（iii）IgA-抗PT应仅用于不确定的IgG-抗PT水平或当无法获得第二个样本。该组不鼓励使用：（i）常规诊断中的其他抗原，因为它们不是特异性的;（ii）微量凝集，因为其缺乏灵敏度;（iii）用于百日咳血清诊断的免疫印迹，因为结果不能量化;（iv）其他方法，例如补体固定或间接免疫荧光，因为它们的低灵敏度和/或特异性。
Bordetella pertussis-specific antibodies can be detected by enzyme-linked immunosorbent assays (ELISAs) or multiplex immunoassays. Assays use purified or mixed antigens, and only pertussis toxin (PT) is specific for B. pertussis. The interpretation of results can be based on dual-sample or single-sample serology using one or two cut-offs. The EU Pertstrain group recommends that: (i) ELISAs and multiplex immunoassays should use purified non-detoxified PT as an antigen, that they should have a broad linear range and that they should express results quantitatively in International Units per millilitre (IU/ml); (ii) a single or dual diagnostic cut-off for single-serum serology using IgG-anti-PT between 50 and 120 IU/ml should be used, and diagnostic serology cannot be validly interpreted for one year after vaccination with acellular pertussis (aP) vaccines; (iii) IgA-anti-PT should only be used with indeterminate IgG-anti-PT levels or when a second sample cannot be obtained. This group discourages using: (i) other antigens in routine diagnostics, as they are not specific; (ii) micro-agglutination, due to its lack of sensitivity; (iii) immunoblots for pertussis serodiagnosis, as results cannot be quantified; (iv) other methods, such as complement fixation or indirect immunofluorescence, due to their low sensitivity and/or specificity.
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DOI：
10.1128/cdli.5.2.130-134.1998
发表时间：
1998-03-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Simondon, F;Iteman, I;Guiso, N
通讯作者：
Guiso, N
影响因子：
4.2
作者：
Versteegh, FGA;Mertens, PLJM;Teunis, PFM
通讯作者：
Teunis, PFM
影响因子：
3
作者：
Litt, David J.;Samuel, Dhanraj;Harrison, Timothy G.
通讯作者：
Harrison, Timothy G.
影响因子：
--
作者：
Xing, Dorothy;von Koenig, Carl Heinz Wirsing;Gaines-Das, Rose
通讯作者：
Gaines-Das, Rose
影响因子：
11.8
作者：
Ward, Joel I.;Cherry, James D.;Edelman, Robert
通讯作者：
Edelman, Robert