Light-Activated Membrane Transport in Polymeric Cell-Mimics.
Light-Activated Membrane Transport in Polymeric Cell-Mimics.
复制标题
聚合细胞模拟中的光激活膜转运。
DOI：
10.1002/anie.202205266
复制
发表时间：
2022-08-22
影响因子：
16.6
通讯作者：
Landfester, Katharina
中科院分区：
文献类型：
--
作者：
Cao, Shoupeng;da Silva, Lucas Caire;Landfester, Katharina
巨型聚合物囊泡是多功能和稳定的仿生隔室，是构建细胞样系统的理想选择。然而，控制细胞样系统功能的亲水性分子跨膜转运受到聚合物双层低渗透性的限制。因此，控制聚合物囊泡渗透性的机制对于创建功能性细胞样系统是必要的。在这里，我们描述了配备有基于螺吡喃的渗透性调节剂的巨型聚合物囊泡的设计。调节剂的光异构化导致聚合物膜的扰动，导致渗透性增加。光活化聚合物囊泡用于构建两个由亲水性分子的光活化转运控制的细胞样系统。首先，我们设计了一个由光照射激活的酶微反应器。其次，我们构建了一种混合凝聚体-聚合物体系统，模拟细胞中生物凝聚体的适应性形成。已经开发出了表现出光驱动适应行为的聚合物细胞模拟物。整合到巨型聚合物囊泡中的基于螺吡喃的调节剂控制了细胞模拟物对外部分子信号的渗透性。光活化的聚合物囊泡被用来创建仿生反应器和系统，模仿自然细胞中生物冷凝物的适应性形成。
Giant polymersomes are versatile and stable biomimetic compartments that are ideal for building cell‐like systems. However, the transport of hydrophilic molecules across the membrane, which controls the function of cell‐like systems, is limited by the low permeability of polymeric bilayers. Therefore, mechanisms to control the permeability of polymersomes are necessary to create functional cell‐like systems. Here, we describe the design of giant polymersomes equipped with spiropyran‐based permeability modulators. Photo‐isomerization of the modulators leads to perturbation of the polymer membrane, resulting in increased permeability. The photoactivated polymersomes were used to construct two cell‐like systems controlled by light‐activated transport of hydrophilic molecules. First, we designed an enzymatic micro‐reactor activated by light irradiation. Second, we constructed a hybrid coacervate‐in‐polymersome system that mimics the adaptive formation of biological condensates in cells. Polymeric cell‐mimics have been developed that exhibit light‐driven adaptive behavior. Spiropyran‐based modulators integrated into giant polymersomes controlled the permeability of the cell‐mimics to external molecular signals. The photoactivated polymersomes were used to create biomimetic reactors and systems that mimic the adaptive formation of biological condensates in natural cells.
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影响因子：
15.1
作者：
Einfalt, Tomaz;Garni, Martina;Palivan, Cornelia G.
通讯作者：
Palivan, Cornelia G.
影响因子：
16.6
作者：
Deshpande, Siddharth;Brandenburg, Frank;Dekker, Cees
通讯作者：
Dekker, Cees
影响因子：
3.4
作者：
Houbrechts, Mazarine;Caire da Silva, Lucas;Landfester, Katharina
通讯作者：
Landfester, Katharina
影响因子：
16.6
作者：
Altenburg WJ;Yewdall NA;Vervoort DFM;van Stevendaal MHME;Mason AF;van Hest JCM
通讯作者：
van Hest JCM
影响因子：
16.6
作者：
Hindley JW;Elani Y;McGilvery CM;Ali S;Bevan CL;Law RV;Ces O
通讯作者：
Ces O