Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China.
Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China.
复制标题
DOI：
10.1186/s12917-018-1374-7
复制
发表时间：
2018-03-02
影响因子：
2.6
通讯作者：
Han B
中科院分区：
文献类型：
--
作者：
Zhang L;Gao J;Barkema HW;Ali T;Liu G;Deng Y;Naushad S;Kastelic JP;Han B
Staphylococcus aureus, a common cause of bovine mastitis, is known for its ability to acquire to antimicrobial resistance and to secrete numerous virulence factors that can exacerbate inflammation. In addition, alpha-hemolysin has an important role in S. aureus infections, diversity of the hla gene (that produces alpha-hmolysin) in S. aureus isolated from bovine mastitis has not been well characterized. The objective was, therefore, to determine diversity of virulence genes, hla gene sequences, and clonal profiles of S. aureus from bovine mastitis in Chinese dairy herds, and to evaluate inter-relationships. The antimicrobials resistance varies from as low as 1.9% (2/103) for CTX to as high as 76.7% (79/103) for penicilin in the 103 isolates and 46 (44.7%) S. aureus were determined as multi-resistant isolates with diverse resistance patterns. Thirty-eight virulence gene patterns (with variable frequencies) were identified in the 103 isolates and correlated with MLST types, indicating a great diversity. Although the hla gene also had great diversity (14 genotypes), Hla peptides were relatively more conserved. With 7 clonal complexes identified from 24 spa types and 7 MLST types. Regarding the letter, ST 97 was the dominant type in S. aureus from bovine mastitis in China. Furthermore, based on phylogenetic analysis, there was a distinct evolutionary relationship between the hla gene and MLST. Multi-resistant S. aureus occurred in bovine mastitis with diverse resistance patterns. The diversity of virulence gene profiles, especially the hla gene and, their relationship with molecular types were reported for the first time in S. aureus from bovine mastitis, which will be useful for future studies on immunogenicity and vaccine development. In addition, based on the distinct evolutionary relationship between the hla gene and MLST types, we inferred that the hla gene has potential role for molecular typing of S. aureus. The online version of this article (10.1186/s12917-018-1374-7) contains supplementary material, which is available to authorized users.
登录
查看更多内容
2014-2015年牛乳腺炎金黄色葡萄球菌分子特征分析
影响因子：
5.7
作者：
Li T;Lu H;Wang X;Gao Q;Dai Y;Shang J;Li M
通讯作者：
Li M
一项为期 5 年的调查显示，中国烧伤中心的金黄色葡萄球菌菌血症患者对耐甲氧西林金黄色葡萄球菌的糖肽敏感性增加
影响因子：
5.2
作者：
Jiang B;Yin S;You B;Huang G;Yang Z;Zhang Y;Chen Y;Chen J;Yuan Z;Rao X;Hu X;Gong Y;Peng Y
通讯作者：
Peng Y
影响因子：
3.8
作者：
Haubert, Louise;Kroning, Isabela Schneid;da Silva, Wladimir Padilha
通讯作者：
da Silva, Wladimir Padilha
影响因子：
3.4
作者：
Miller, Martin Lee;Soufi, Boumediene;Mijakovic, Ivan
通讯作者：
Mijakovic, Ivan
影响因子：
5.3
作者：
Koziel, Joanna;Chmiest, Daniela;Potempa, Jan
通讯作者：
Potempa, Jan