The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model.
The severity of LPS induced inflammatory injury is negatively associated with the functional liver mass after LPS injection in rat model.
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DOI：
10.1186/s12950-018-0197-4
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Dahmen U
中科院分区：
文献类型：
--
作者：
Fang H;Liu A;Chen X;Cheng W;Dirsch O;Dahmen U
脓毒症患者血清脂多糖（LPS）水平高，伴有肝损伤，死亡率高。然而，肝脏在调节LPS诱导的炎症损伤中的作用尚不清楚。在本研究中，观察了肝切除或门静脉分支阻断以减少功能性肝质量后LPS诱导的炎症反应的严重程度。观察局部和全身的损伤情况，探讨肝脏在炎症损伤中的调节作用。分别行30%、70%和90%肝部分切除术（PH），观察血清TNF-α、存活率和肝脏LPS摄取情况。第二，在注射LPS之前选择性阻断RL，降低了功能性肝脏质量的LPS暴露，在70%PH之前30分钟给予RL，并比较了闭塞和非闭塞肝脏中的炎症反应。对照组在肝切除前30 min注射LPS，不短暂阻断RL。观察血清TNF-α、ALT、AST、肌酐、尿素水平、存活率、肝内脂多糖（LPS）摄取及肝脏炎症细胞因子的变化。90%、70%和30%PH后功能性肝质量的减少与血清TNF-α、存活率的降低以及LPS注射后肝内LPS摄取的增加有关。在LPS给药前阻断右叶（RL）逆转了由70%PH引起的肝损伤，表现为100%的存活率和降低的肝和肾损伤以及全身炎症反应。闭塞肝叶的炎症反应诱导率低于未闭塞肝叶。LPS诱导的全身性炎性损伤的严重程度由功能性肝体积决定。这一观察结果表明，肝脏是引发炎症反应的中心器官，并参与引起严重的SIRS，伴有全身性损伤和死亡。本文的在线版本（10.1186/s12950-018-0197-4）包含补充材料，可供授权用户使用。
High levels of serum lipopolysaccharide (LPS) were observed in sepsis patients with liver injury and high mortality. However, the role of liver in modulation LPS induced inflammatory injury was ill investigated. In the present study, the severity of LPS induced inflammatory response was observed after liver resection or portal branch occlusion to decreasing functional liver mass. The local and systemic damage was observed to investigate the role of liver in modulation inflammatory injury. First, 30%, 70%, and 90% partial hepatectomy (PH) were performed, and serum TNF-α, survival rate, and hepatic LPS uptake was observed. Second, LPS-exposure of the functional liver mass was decreased by selectively blocking the RL prior to LPS-injection, which was given 30 min before a 70% PH, and the inflammatory response was compared in the occluded and the non-occluded liver. The control group was subjected to LPS injection 30 min prior to liver resection without blocking the RL transiently. The serum TNF-α, ALT, AST, creatinine levels, and urea levels, survival rate, hepatic LPS uptake, and hepatic inflammatory cytokines was observed. The decreasing of functional liver mass after 90%, 70%, and 30% PH was associated with decreased serum TNF-α, survival rate, and increased hepatic LPS uptake after LPS injection. Occluding the right lobes (RL) prior to LPS administration reversed the liver injury caused by 70% PH, indicated by 100% survival rate and decreased liver and kidney injury, and systemic inflammatory response. The induction of inflammatory response in occluding liver lobes were lower than un-occluding liver lobes. The severity of the LPS-induced systemic inflammatory injury is determined by functional liver volume. This observation suggests that the liver is the central organ for the initiation of the inflammatory response, and is involved in causing a severe SIRS with systemic damage and death. The online version of this article (10.1186/s12950-018-0197-4) contains supplementary material, which is available to authorized users.
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影响因子：
5
作者：
Yan J;Li S;Li S
通讯作者：
Li S
粒细胞落刺激因子通过上调大鼠的LPS结合蛋白来诱导脂多糖（LPS）敏化。
影响因子：
3.7
作者：
Fang H;Liu A;Sun J;Kitz A;Dirsch O;Dahmen U
通讯作者：
Dahmen U
影响因子：
5.2
作者：
Ramachandran G
通讯作者：
Ramachandran G
DOI：
10.1164/ajrccm.149.1.8111596
发表时间：
1994-01-01
影响因子：
24.7
作者：
MATUSCHAK, GM;MATTINGLY, ME;LECHNER, AJ
通讯作者：
LECHNER, AJ
影响因子：
5.2
作者：
Fink MP
通讯作者：
Fink MP