Positive crosstalk of MAMP signaling pathways in rice cells.
Positive crosstalk of MAMP signaling pathways in rice cells.
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DOI：
10.1371/journal.pone.0051953
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Shibuya N
中科院分区：
文献类型：
--
作者：
Desaki Y;Otomo I;Kobayashi D;Jikumaru Y;Kamiya Y;Venkatesh B;Tsuyumu S;Kaku H;Shibuya N
植物已经进化出了高效的防御机制，即启动和协同机制，这两种机制都可以更广泛地动员防御反应，以抵御连续的病原体入侵或不同信号分子的同时刺激。然而，这些现象背后的机制在很大程度上是未知的。在本研究中，我们以培养的水稻细胞和纯化的MAMP分子组合作为模型系统来研究这些现象的机制。我们发现，低浓度细菌脂多糖（LPS）预处理水稻细胞明显启动了连续处理n -乙酰壳聚糖（GN8）诱导的防御反应。另一方面，GN8和LPS同时处理也导致了类似的LPS诱导的防御反应增强，这表明这些MAMPs的协同作用基本上是防御反应增强的原因，尽管在某些实验条件下这种作用可以解释为“启动”。这些结果也表明，MAMP受体下游信号级联的这种正串扰似乎发生得非常迅速，可能发生在信号通路的早期阶段。对植物激素的综合分析显示，在LPS预处理和同时处理中，茉莉酸（jasmonic acid， JA）的合成都有特异性的增强，这表明JA在增强下游反应中发挥了作用。
Plants have evolved efficient defense mechanisms known as priming and synergy, both of which can mobilize defense responses more extensively against successive pathogen invasion or simultaneous stimulation by different signal molecules. However, the mechanisms underlying these phenomena were largely unknown. In the present study, we used cultured rice cells and combination of purified MAMP molecules as a model system to study the mechanisms of these phenomena. We found that the pretreatment of rice cells with a low concentration of bacterial lipopolysaccharide (LPS) apparently primed the defense responses induced by successive N-acetylchitooctaose (GN8) treatment. On the other hand, simultaneous treatment with GN8 and LPS also resulted in the similar enhancement of defense responses observed for the LPS-induced priming, indicating that the synergistic effects of these MAMPs are basically responsible for such enhancement of defense responses, though the effect could be interpreted as “priming” under some experimental conditions. These results also indicate that such a positive crosstalk of signaling cascade downstream of MAMP receptors seems to occur very rapidly, probably at early step(s) of signaling pathway. Comprehensive analysis of phytohormones revealed a specific enhancement of the synthesis of jasmonic acid (JA), both in the LPS pretreatment and also simultaneous treatment, indicating a role of JA in the enhancement of downstream responses.
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影响因子：
3.6
作者：
Makela, Sanna M.;Osterlund, Pamela;Julkunen, Ilkka
通讯作者：
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影响因子：
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作者：
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通讯作者：
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