Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells.
Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells.
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拉曼光谱学发现了来自间充质基质细胞的细胞外囊泡的生化组织相关特征。
DOI：
10.1038/s41598-017-10448-1
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发表时间：
2017-08-29
影响因子：
4.6
通讯作者：
Gramatica F
中科院分区：
文献类型：
--
作者：
Gualerzi A;Niada S;Giannasi C;Picciolini S;Morasso C;Vanna R;Rossella V;Masserini M;Bedoni M;Ciceri F;Bernardo ME;Brini AT;Gramatica F
来自间充质基质细胞（MSC）的细胞外囊泡（EV）正在成为用于组织再生和免疫调节的有价值的治疗剂，但其临床应用迄今为止受到当前分离和表征程序的技术限制。本研究首次成功应用拉曼光谱法，在体外或体内使用前，对MSC衍生囊泡进行常规批量表征，无需标记，灵敏且可重复，从而促进EV研究向临床实践的转化。将骨髓和脂肪组织来源的MSC的EV的拉曼光谱与人真皮成纤维细胞EV进行比较，以证明该方法自动区分三种细胞型的囊泡的能力，准确度为93.7%。我们的数据属性的拉曼指纹电动汽车从不同组织来源的未分化和分化的细胞，并提供洞察电动汽车的生化特征，从不同的来源和到不同的贡献鞘磷脂，神经节苷脂和磷脂酰胆碱的拉曼光谱本身。
Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice. The Raman spectra of the EVs of bone marrow and adipose tissue-derived MSCs were compared with human dermal fibroblast EVs in order to demonstrate the ability of the method to distinguish the vesicles of the three cytotypes automatically with an accuracy of 93.7%. Our data attribute a Raman fingerprint to EVs from undifferentiated and differentiated cells of diverse tissue origin, and provide insights into the biochemical characteristics of EVs from different sources and into the differential contribution of sphingomyelin, gangliosides and phosphatidilcholine to the Raman spectra themselves.
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