Differences in grain ultrastructure, phytochemical and proteomic profiles between the two contrasting grain Cd-accumulation barley genotypes.
Differences in grain ultrastructure, phytochemical and proteomic profiles between the two contrasting grain Cd-accumulation barley genotypes.
复制标题
DOI：
10.1371/journal.pone.0079158
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wu F
中科院分区：
文献类型：
--
作者：
Sun H;Cao F;Wang N;Zhang M;Mosaddek Ahmed I;Zhang G;Wu F
为了揭示两个大麦基因型之间的籽粒理化和蛋白质组差异，对籽粒 Cd 高积累和低积累的哲农 8 号和 W6nk2 之间的籽粒超微结构、氨基酸和蛋白质特征进行了比较。结果表明，与浙农8号相比，W6nk2的蛋白质含量显着降低，其中大麦醇溶蛋白基因型差异最大，氨基酸含量也较低，尤其是Glu、Tyr、Phe、Pro比例较低。扫描和透射电镜观察均表明W6nk2中A型淀粉分子的尺寸明显大于浙农8号。浙农8号籽粒在淀粉颗粒周围有较多富含蛋白质的沉积物，部分A型颗粒表面有凹坑。通过2-DE结合质谱分析，在谷物中鉴定出17个蛋白质，其中Zhenong8中的表达量高于W6nk2；包括z型丝氨酸蛋白酶抑制剂、丝氨酸蛋白酶抑制剂-Z7和α-淀粉酶/胰蛋白酶抑制剂CM、碳水化合物代谢、蛋白质合成和信号转导相关蛋白质。浙农8中有12个蛋白的表达量低于W6nk2；包括大麦胰蛋白酶抑制剂氯仿/甲醇可溶性蛋白（BTI-CMe2.1、BTI-CMe2.2）、胰蛋白酶抑制剂、脱氢抗坏血酸还原酶（DHAR）、周中心蛋白、动力蛋白重链和一些抗病毒相关蛋白。这些数据扩展了我们对镉积累/耐受性机制的理解，并为开发低粒镉大麦品种提供了利用优良遗传资源的可能。
To reveal grain physio-chemical and proteomic differences between two barley genotypes, Zhenong8 and W6nk2 of high- and low- grain-Cd-accumulation, grain profiles of ultrastructure, amino acid and proteins were compared. Results showed that W6nk2 possesses significantly lower protein content, with hordein depicting the greatest genotypic difference, compared with Zhenong8, and lower amino acid contents with especially lower proportion of Glu, Tyr, Phe and Pro. Both scanning and transmission electron microscopy observation declared that the size of A-type starch molecule in W6nk2 was considerably larger than that of Zhenong8. Grains of Zhenong8 exhibited more protein-rich deposits around starch granules, with some A-type granules having surface pits. Seventeen proteins were identified in grains, using 2-DE coupled with mass spectrometry, with higher expression in Zhenong8 than that in W6nk2; including z-type serpin, serpin-Z7 and alpha-amylase/trypsin inhibitor CM, carbohydrate metabolism, protein synthesis and signal transduction related proteins. Twelve proteins were less expressed in Zhenong8 than that in W6nk2; including barley trypsin inhibitor chloroform/methanol-soluble protein (BTI-CMe2.1, BTI-CMe2.2), trypsin inhibitor, dehydroascorbate reductase (DHAR), pericentrin, dynein heavy chain and some antiviral related proteins. The data extend our understanding of mechanisms underlying Cd accumulation/tolerance and provides possible utilization of elite genetic resources in developing low-grain-Cd barley cultivars.
登录
查看更多内容
影响因子：
6.4
作者：
KIRSI, M
通讯作者：
KIRSI, M
影响因子：
6.9
作者：
Forster, BP;Ellis, RP;Ben Salem, M
通讯作者：
Ben Salem, M
影响因子：
5.4
作者：
Liu, X;Yang, J;Frey, TK
通讯作者：
Frey, TK
影响因子：
3.1
作者：
PENNER, GA;CLARKE, J;LEISLE, D
通讯作者：
LEISLE, D
籽粒镉低积累大麦基因型的鉴定及其与四种微量元素的相互作用。
影响因子：
8.8
作者：
Chen, Fei;Dong, Jing;Wei, Kang
通讯作者：
Wei, Kang