Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
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DOI：
10.1038/msb4100049
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发表时间：
2006
影响因子：
9.9
通讯作者：
中科院分区：
文献类型：
--
作者：
为了解决以大肠杆菌K-12为模型细胞的全细胞问题，确定了两种密切相关的K-12菌株MG 1655和W3110的高度精确的基因组。完成W3110基因组并与MG 1655基因组进行比较，发现在267个位点存在差异，包括251个具有短的、主要是单核苷酸的插入或缺失（indels）或碱基取代（总共358个核苷酸）的位点，此外还有13个位点具有插入序列元件或仅在一个菌株中存在缺陷的前噬菌体，以及两个W3110倒位位点。对具有短插入缺失和碱基差异的251个区域的PCR产物进行直接DNA测序，发现只有8个位点是真正的差异。其他243个差异是由于原始MG 1655序列中的错误，包括79个移码，1个氨基酸残基缺失，5个氨基酸残基插入，73个错义和17个编码区内的沉默变化。原始MG 1655序列中的错误（<1/13000碱基）大多位于基于放射性化学的过时技术测序的部分内。
With the goal of solving the whole-cell problem with Escherichia coli K-12 as a model cell, highly accurate genomes were determined for two closely related K-12 strains, MG1655 and W3110. Completion of the W3110 genome and comparison with the MG1655 genome revealed differences at 267 sites, including 251 sites with short, mostly single-nucleotide, insertions or deletions (indels) or base substitutions (totaling 358 nucleotides), in addition to 13 sites with an insertion sequence element or defective prophage in only one strain and two sites for the W3110 inversion. Direct DNA sequencing of PCR products for the 251 regions with short indel and base disparities revealed that only eight sites are true differences. The other 243 discrepancies were due to errors in the original MG1655 sequence, including 79 frameshifts, one amino-acid residue deletion, five amino-acid residue insertions, 73 missense, and 17 silent changes within coding regions. Errors in the original MG1655 sequence (<1 per 13 000 bases) were mostly within portions sequenced with out-dated technology based on radioactive chemistry.
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影响因子：
56.9
作者：
DANIELS, DL;PLUNKETT, G;BLATTNER, FR
通讯作者：
BLATTNER, FR
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.2
作者：
Jishage, M;Ishihama, A
通讯作者：
Ishihama, A
影响因子：
64.5
作者：
KOHARA, Y;AKIYAMA, K;ISONO, K
通讯作者：
ISONO, K