Expression of the Malus sieversii NF-YB21 Encoded Gene Confers Tolerance to Osmotic Stresses in Arabidopsis thaliana.
Expression of the Malus sieversii NF-YB21 Encoded Gene Confers Tolerance to Osmotic Stresses in Arabidopsis thaliana.
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DOI：
10.3390/ijms22189777
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发表时间：
2021-09-10
影响因子：
5.6
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Feng C;Wang Y;Sun Y;Peng X;Zhang X;Zhou X;Jiao J;Zhai Z;Xiao Y;Wang W;Liu Y;Li T
干旱是限制干旱半干旱地区苹果产量和品质的主要环境因子。核因子Ys（Nuclear Factor Ys，NF-Ys）是一类重要的转录因子，参与植物生长发育和各种逆境胁迫反应的调控。然而，NF-Y基因在苹果中的功能知之甚少。在这里，我们确定了苹果基因组中的43个NF-Y基因，并对苹果NF-Y进行了初步的功能表征。M. sieversii揭示，与叶子相比，大量NF-Y在根中高度表达，并且很大一部分NF-Y基因对干旱处理有反应。此外，MsNF-YB 21的异源表达，这是显着上调干旱，导致更长的根长，因此，提高了拟南芥的渗透和耐盐性。此外，MsNF-YB 21过表达的生理分析揭示了增强的抗氧化系统，包括抗氧化酶和相容性溶质。此外，编码过氧化氢酶（AtCAT 2、AtCAT 3）、超氧化物歧化酶（AtFSD 1、AtFSD 3、AtCSD 1）和过氧化物酶（AtPER 12、AtPER 42、AtPER 47、AtPER 51）的基因在MsNF-YB 21过表达系中表现出上调表达。MsNF-Y基因家族的研究结果为苹果NF-Y基因的进一步研究提供了有用的信息，MsNF-YB 21的功能分析支持其作为通过生物技术改良苹果耐旱性的潜在靶标。
Drought is the main environmental factor that limits the yield and quality of apples (Malus × domestica) grown in arid and semi-arid regions. Nuclear factor Ys (NF-Ys) are important transcription factors involved in the regulation of plant growth, development, and various stress responses. However, the function of NF-Y genes is poorly understood in apples. Here, we identified 43 NF-Y genes in the genome of apples and conducted an initial functional characterization of the apple NF-Y. Expression analysis of NF-Y members in M. sieversii revealed that a large number of NF-Ys were highly expressed in the roots compared with the leaves, and a large proportion of NF-Y genes responded to drought treatment. Furthermore, heterologous expression of MsNF-YB21, which was significantly upregulated by drought, led to a longer root length and, thus, conferred improved osmotic and salt tolerance in Arabidopsis. Moreover, the physiological analysis of MsNF-YB21 overexpression revealed enhanced antioxidant systems, including antioxidant enzymes and compatible solutes. In addition, genes encoding catalase (AtCAT2, AtCAT3), superoxide dismutase (AtFSD1, AtFSD3, AtCSD1), and peroxidase (AtPER12, AtPER42, AtPER47, AtPER51) showed upregulated expression in the MsNF-YB21 overexpression lines. These results for the MsNF-Y gene family provide useful information for future studies on NF-Ys in apples, and the functional analysis of MsNF-YB21 supports it as a potential target in the improvement of apple drought tolerance via biotechnological strategies.
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苹果核因子-Y 基因家族的基因组组织、系统发育比较和差异表达
DOI：
10.3390/plants10010016
发表时间：
2020-12-24
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Qu Y;Wang Y;Zhu J;Zhang Y;Hou H
通讯作者：
Hou H
DOI：
10.1073/pnas.0437909100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Lee, HS;Fischer, RL;Harada, JJ
通讯作者：
Harada, JJ
影响因子：
3.7
作者：
Leyva-González MA;Ibarra-Laclette E;Cruz-Ramírez A;Herrera-Estrella L
通讯作者：
Herrera-Estrella L
影响因子：
13.8
作者：
Chen, Ming;Sun, Liying;Xu, Huijun
通讯作者：
Xu, Huijun
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF