Functional and transcriptome analysis reveals an acclimatization strategy for abiotic stress tolerance mediated by Arabidopsis NF-YA family members.
Functional and transcriptome analysis reveals an acclimatization strategy for abiotic stress tolerance mediated by Arabidopsis NF-YA family members.
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DOI：
10.1371/journal.pone.0048138
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Herrera-Estrella L
中科院分区：
文献类型：
--
作者：
Leyva-González MA;Ibarra-Laclette E;Cruz-Ramírez A;Herrera-Estrella L
核因子Y(NF-Y)是由核因子-YA/核因子-YB/核因子-YC亚基与真核启动子中的CCAAT盒结合形成的异源三聚体复合体。与其他生物体中每个亚基由一个基因编码的情况不同，在植物中，超过10个成员的基因家族对每个亚基进行编码。在这里，我们报道了拟南芥NF-YA家族的五个成员通过转录和miR169相关的转录后机制在几种胁迫条件下强烈诱导。过表达NF-YA2、7和10导致矮化晚衰老植株对几种类型的非生物胁迫的耐受性增强。这些表型与在过量表达NF-YA植物中观察到的蔗糖/淀粉平衡和细胞伸长的变化有关。对在雌二醇诱导系统下表达miR169抗性版本的NF-YA2、3、7和10的转基因植物以及显性-阻遏版本的NF-YA2进行转录转录分析，揭示了一组基因，其启动子富含NF-Y结合位点(CCAAT-box)，可能直接受NF-Y复合体的调控。这一分析还表明，NF-Yas可能通过正、负两种机制参与基因调控。我们提出了一个模型，在该模型中，对非生物胁迫的反应中，NF-YA转录水平的增加是对不利环境条件的适应性反应的一部分，而植物生长速度的降低在其中起着关键作用。
Nuclear Factor Y (NF-Y) is a heterotrimeric complex formed by NF-YA/NF-YB/NF-YC subunits that binds to the CCAAT-box in eukaryotic promoters. In contrast to other organisms, in which a single gene encodes each subunit, in plants gene families of over 10 members encode each of the subunits. Here we report that five members of the Arabidopsis thaliana NF-YA family are strongly induced by several stress conditions via transcriptional and miR169-related post-transcriptional mechanisms. Overexpression of NF-YA2, 7 and 10 resulted in dwarf late-senescent plants with enhanced tolerance to several types of abiotic stress. These phenotypes are related to alterations in sucrose/starch balance and cell elongation observed in NF-YA overexpressing plants. The use of transcriptomic analysis of transgenic plants that express miR169-resistant versions of NF-YA2, 3, 7, and 10 under an estradiol inducible system, as well as a dominant-repressor version of NF-YA2 revealed a set of genes, whose promoters are enriched in NF-Y binding sites (CCAAT-box) and that may be directly regulated by the NF-Y complex. This analysis also suggests that NF-YAs could participate in modulating gene regulation through positive and negative mechanisms. We propose a model in which the increase in NF-YA transcript levels in response to abiotic stress is part of an adaptive response to adverse environmental conditions in which a reduction in plant growth rate plays a key role.
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影响因子：
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作者：
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