A structural analysis of M protein in coronavirus assembly and morphology.
A structural analysis of M protein in coronavirus assembly and morphology.
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DOI：
10.1016/j.jsb.2010.11.021
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发表时间：
2011-04
影响因子：
3
通讯作者：
Buchmeier, Michaell J.
中科院分区：
文献类型：
--
作者：
Neuman, Benjamin W.;Kiss, Gabriella;Kunding, Andreas H.;Bhella, David;Baksh, M. Fazil;Connelly, Stephen;Droese, Ben;Klaus, Joseph P.;Makino, Shinji;Sawicki, Stanley G.;Siddell, Stuart G.;Stamou, Dimitrios G.;Wilson, Ian A.;Kuhn, Peter;Buchmeier, Michaell J.
关键词：
冠状病毒的M蛋白在病毒组装中起着核心作用，它将细胞膜变成了病毒和宿主因子聚集在一起制造新病毒颗粒的车间。我们研究了M结构和组织是如何与病毒的形状和大小，使用冷冻电子显微镜，断层扫描和统计分析。我们目前的证据表明，M可以采用两种构象和膜曲率调节一个M构象。伸长的M蛋白与刚性、簇状峰和相对窄的膜曲率范围相关。相比之下，紧凑M蛋白与灵活性和低峰密度相关。对几种类型的病毒样颗粒和病毒体的分析表明，S蛋白、N蛋白和基因组RNA都有助于调节病毒体的大小和变异，推测是通过与M.这些发现为了解M蛋白如何促进病毒组装提供了线索。
The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.
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DOI：
10.1016/j.str.2005.08.021
发表时间：
2005-12
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Fan H;Ooi A;Tan YW;Wang S;Fang S;Liu DX;Lescar J
通讯作者：
Lescar J
影响因子：
5.4
作者：
de Haan, CAM;Vennema, H;Rottier, PJM
通讯作者：
Rottier, PJM
影响因子：
5.4
作者：
GALLAGHER, TM;ESCARMIS, C;BUCHMEIER, MJ
通讯作者：
BUCHMEIER, MJ
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
2.9
作者：
Huang, QL;Yu, LP;Olejniczak, ET
通讯作者：
Olejniczak, ET