Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus.
Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus.
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类胡萝卜素积累影响柑橘中的氧化还原状态、淀粉代谢和类黄酮/花青素积累
DOI：
10.1186/s12870-015-0426-4
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发表时间：
2015-02-03
影响因子：
5.3
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Cao H;Wang J;Dong X;Han Y;Ma Q;Ding Y;Zhao F;Zhang J;Chen H;Xu Q;Xu J;Deng X
类胡萝卜素是植物不可缺少的次生代谢产物，参与光合作用、抗氧化和植物激素的生物合成。类胡萝卜素可能还参与了其他尚未被发现的生物功能。在这项研究中，我们整合了基因组、生化和细胞研究，以深入了解柑橘愈伤组织中过表达CrtB （Pantoea agglomerans的植物烯合成酶）的类胡萝卜素相关生物学过程。此外，还以柑橘近亲Fortunella hindsii Swingle和野生苹果Malus hupehensis愈伤组织为支撑体系，研究了类胡萝卜素积累改变对类胡萝卜素相关生物过程的影响。转录组学分析提供了对类胡萝卜素相关的氧化还原状态、淀粉代谢和类黄酮/花青素积累的生物学过程的深入了解。通过生化和细胞学分析，我们确定氧化还原状态的改变与O2-和H2O2水平的变化有关。我们还确定了富含类胡萝卜素的愈伤组织中淀粉积累的减少。此外，通过对新构建的过表达Fortunella hindsii Swingle的CrtB进行广泛的细胞研究，我们证明淀粉水平的降低与显著的类胡萝卜素积累平行发生。此外，对富含花青素的海参愈伤组织的研究表明，类胡萝卜素对花青素积累有负面影响。在柑橘中，类胡萝卜素积累的改变对氧化还原修饰、淀粉降解和类黄酮/花青素生物合成等代谢过程产生了显著影响。这些发现为理解胡萝卜素生成的生物学重要性以及与积累类胡萝卜素的农产品的营养和感官品质相关的发育过程提供了新的视角。本文的在线版本（doi:10.1186/s12870-015-0426-4）包含补充材料，可供授权用户使用。
Carotenoids are indispensable plant secondary metabolites that are involved in photosynthesis, antioxidation, and phytohormone biosynthesis. Carotenoids are likely involved in other biological functions that have yet to be discovered. In this study, we integrated genomic, biochemical, and cellular studies to gain deep insight into carotenoid-related biological processes in citrus calli overexpressing CrtB (phytoene synthase from Pantoea agglomerans). Fortunella hindsii Swingle (a citrus relative) and Malus hupehensis (a wild apple) calli were also utilized as supporting systems to investigate the effect of altered carotenoid accumulation on carotenoid-related biological processes. Transcriptomic analysis provided deep insight into the carotenoid-related biological processes of redox status, starch metabolism, and flavonoid/anthocyanin accumulation. By applying biochemical and cytological analyses, we determined that the altered redox status was associated with variations in O2- and H2O2 levels. We also ascertained a decline in starch accumulation in carotenoid-rich calli. Furthermore, via an extensive cellular investigation of the newly constructed CrtB overexpressing Fortunella hindsii Swingle, we demonstrated that starch level reducation occurred in parallel with significant carotenoid accumulation. Moreover, studying anthocyanin-rich Malus hupehensis calli showed a negative effect of carotenoids on anthocyanin accumulation. In citrus, altered carotenoid accumulation resulted in dramatic effects on metabolic processes involved in redox modification, starch degradation, and flavonoid/anthocyanin biosynthesis. These findings provided new perspectives to understand the biological importance of carotenogenesis and of the developmental processes associated with the nutritional and sensory qualities of agricultural products that accumulate carotenoids. The online version of this article (doi:10.1186/s12870-015-0426-4) contains supplementary material, which is available to authorized users.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
11.6
作者：
Cazzonelli, Christopher I.;Cuttriss, Abby J.;Pogson, Barry J.
通讯作者：
Pogson, Barry J.
影响因子：
7.4
作者：
Diretto, Gianfranco;Al-Babili, Salim;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
影响因子：
5.1
作者：
Fuentes, Paulina;Pizarro, Lorena;Stange, Claudia
通讯作者：
Stange, Claudia
影响因子：
7.2
作者：
Bai, Chao;Rivera, Sol M.;Zhu, Changfu
通讯作者：
Zhu, Changfu