Mapping QTL for Seed Germinability under Low Temperature Using a New High-Density Genetic Map of Rice.
Mapping QTL for Seed Germinability under Low Temperature Using a New High-Density Genetic Map of Rice.
复制标题
使用新的水稻高密度遗传图谱绘制低温下种子发芽率的 QTL
DOI：
10.3389/fpls.2017.01223
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发表时间：
2017
影响因子：
5.6
通讯作者：
Bian J
中科院分区：
文献类型：
--
作者：
Jiang N;Shi S;Shi H;Khanzada H;Wassan GM;Zhu C;Peng X;Yu Q;Chen X;He X;Fu J;Hu L;Xu J;Ouyang L;Sun X;Zhou D;He H;Bian J
水稻种子低温萌发性主要数量性状位点的定位可以为提高水稻的耐冷性提供有价值的遗传资源。在本研究中，124个水稻回交重组自交系（BRILs）来源于一个杂交籼稻。长恢891和粳稻。通过重测序技术对02428进行基因分型。生成的箱图包含3057个箱，箱距1266.5 cM，平均标记间距0.41 cM。在新构建的高密度遗传图谱的基础上，在Nipponbare基因组上检测到6个长度在40 ~ 140 kb之间的QTL。其中，02428共有的两个QTL qCGR8和qGRR11等位基因分别能提高GULT和冷胁迫后种子萌发复发率。而在正常条件下，qNGR1和qNGR4可能是影响籼稻长恢891萌发的两个主要QTL。QTL qGRR1和qGRR8影响冷胁迫后种子的萌发复发率，长恢891共有的效应增强等位基因能显著提高冷胁迫后种子的萌发率。这些QTL可能是水稻耐寒性改良的重要遗传因子。此外，本研究开发的bril将为水稻复杂性状的定位和遗传基础研究提供合适的选择。
Mapping major quantitative trait loci (QTL) responsible for rice seed germinability under low temperature (GULT) can provide valuable genetic source for improving cold tolerance in rice breeding. In this study, 124 rice backcross recombinant inbred lines (BRILs) derived from a cross indica cv. Changhui 891 and japonica cv. 02428 were genotyped through re-sequencing technology. A bin map was generated which includes 3057 bins covering distance of 1266.5 cM with an average of 0.41 cM between markers. On the basis of newly constructed high-density genetic map, six QTL were detected ranging from 40 to 140 kb on Nipponbare genome. Among these, two QTL qCGR8 and qGRR11 alleles shared by 02428 could increase GULT and seed germination recovery rate after cold stress, respectively. However, qNGR1 and qNGR4 may be two major QTL affecting indica Changhui 891germination under normal condition. QTL qGRR1 and qGRR8 affected the seed germination recovery rate after cold stress and the alleles with increasing effects were shared by the Changhui 891 could improve seed germination rate after cold stress dramatically. These QTL could be a highly valuable genetic factors for cold tolerance improvement in rice lines. Moreover, the BRILs developed in this study will serve as an appropriate choice for mapping and studying genetic basis of rice complex traits.
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影响因子：
4.1
作者：
Da, Y;VanRaden, PM;Schook, LB
通讯作者：
Schook, LB
DOI：
10.1073/pnas.0805303105
发表时间：
2008-08-26
影响因子：
11.1
作者：
Fujino, Kenji;Sekiguchi, Hiroshi;Yano, Masahiro
通讯作者：
Yano, Masahiro
影响因子：
4.4
作者：
Chen Z;Wang B;Dong X;Liu H;Ren L;Chen J;Hauck A;Song W;Lai J
通讯作者：
Lai J
影响因子：
7
作者：
Huang, Xuehui;Feng, Qi;Han, Bin
通讯作者：
Han, Bin
影响因子：
2.4
作者：
Bian, Jian Min;Jiang, Ling;Wan, Jian Min
通讯作者：
Wan, Jian Min