Identification of Drosophila gene products required for phagocytosis of Leishmania donovani.
Identification of Drosophila gene products required for phagocytosis of Leishmania donovani.
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DOI：
10.1371/journal.pone.0051831
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Smith DF
中科院分区：
文献类型：
--
作者：
Peltan A;Briggs L;Matthews G;Sweeney ST;Smith DF
The identity and function of host factors required for efficient phagocytosis and intracellular maintenance of the protozoan parasite Leishmania donovani are poorly understood. Utilising the phagocytic capability of Drosophila S2 cells, together with available tools for modulating gene expression by RNAi, we have developed an experimental system in which to identify host proteins of this type on a genome-wide scale. We have shown that L. donovani amastigotes can be phagocytosed by S2 cells, in which they replicate and are maintained in a compartment with features characteristic of mammalian phagolysosomes. Screening with dsRNAs from 1920 conserved metazoan genes has identified transcripts that, when reduced in expression, cause either increased or decreased phagocytosis. Focussing on genes in the latter class, RNAi-mediated knockdown of the small GTPase Rab5, the prenylated SNARE protein YKT6, one sub-unit of serine palmitoyltransferase (spt2/lace), the Rac1-associated protein Sra1 and the actin cytoskeleton regulatory protein, SCAR, all lead to a significant reduction in parasite phagocytosis. A role for the lace mammalian homologue in amastigote uptake by mammalian macrophages has been verified using the serine palmitoyltransferase inhibitor, myriocin. These observations suggest that this experimental approach has the potential to identify a large number of host effectors required for efficient parasite uptake and maintenance.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.1073/pnas.0506461102
发表时间：
2005-09-20
影响因子：
11.1
作者：
Cheng, LW;Viala, JPM;Portnoy, DA
通讯作者：
Portnoy, DA
影响因子：
64.8
作者：
Bard, F;Casano, L;Malhotra, V
通讯作者：
Malhotra, V
DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
Clemens, JC;Worby, CA;Dixon, JE
通讯作者：
Dixon, JE
影响因子：
10.5
作者：
Baeg, GH;Zhou, R;Perrimon, N
通讯作者：
Perrimon, N