Small fitness effects and weak genetic interactions between deleterious mutations in heterozygous loci of the yeast Saccharomyces cerevisiae.
Small fitness effects and weak genetic interactions between deleterious mutations in heterozygous loci of the yeast Saccharomyces cerevisiae.
复制标题
酿酒酵母杂合位点有害突变之间的适应性效应较小，遗传相互作用较弱。
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
R. Korona
中科院分区：
文献类型：
--
作者：
Krzysztof Szafraniec;D. Wloch;P. Śliwa;R. Borts;R. Korona
Rare, random mutations were induced in budding yeast by ethyl methanesulfonate (EMS). Clones known to bear a single non-neutral mutation were used to obtain mutant heterozygotes and mutant homozygotes that were later compared with wild-type homozygotes. The average homozygous effect of mutation was an approximately 2% decrease in the growth rate. In heterozygotes, the harmful effect of these relatively mild mutations was reduced approximately fivefold. In a test of epistasis, two heterozygous mutant loci were paired at random. Fitness of the double mutants was best explained by multiplicative action of effects at single loci, with little evidence for epistasis and essentially excluding synergism. In other experiments, the same mutations in haploid and heterozygous diploid clones were compared. Regardless of the haploid phenotypes, mildly deleterious or lethal, fitness of the heterozygotes was decreased by less than half a per cent on average. In general, the results presented here suggest that most mutations tend to exhibit small and weakly interacting effects in heterozygous loci regardless of how harmful they are in haploids or homozygotes.
上位选择下的穆勒棘轮。
影响因子：
3.3
作者：
Kondrashov,AS
通讯作者：
Kondrashov,AS
影响因子：
3.3
作者：
Sarah P. Otto;David Goldstein
通讯作者：
Sarah P. Otto;David Goldstein
影响因子：
56.9
作者：
Galitski, T;Saldanha, AJ;Fink, GR
通讯作者：
Fink, GR