State-of-the-Art Techniques to Causally Link Neural Plasticity to Functional Recovery in Experimental Stroke Research.
State-of-the-Art Techniques to Causally Link Neural Plasticity to Functional Recovery in Experimental Stroke Research.
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DOI：
10.1155/2018/3846593
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发表时间：
2018
影响因子：
3.1
通讯作者：
Wahl AS
中科院分区：
文献类型：
--
作者：
Wahl AS
目前的实验性中风研究面临着与神经科学相同的挑战：将相关发现转化为病因研究结果。近年来的研究表明，中枢神经系统对伤害性刺激如中风做出反应的巨大潜力：在临床和实验性中风研究中，已描述了增加的可塑性变化导致神经元重新连接、神经发生和突触形成的形式，并伴随着受影响细胞的转录和翻译周转。然而，只有很少的尝试将不同的塑料重塑过程联系起来，作为特定行为表型的起因特征。在这里，我们回顾了目前最先进的检查皮质重组和操纵神经元回路的技术，以及将解剖学变化与分子图谱相结合的技术。我们提供了这些技术的原理，以及已经应用了所描述的方法的实验性中风研究的研究。所讨论的工具有助于闭合从我们对中风病理的理解到行为结果的循环，并可能为治疗方法发现新的靶点。通过从神经生物学的基础上了解康复策略对内在修复机制的外部影响，这里提出的方法为评估康复策略的效率开辟了新的可能性。
Current experimental stroke research faces the same challenge as neuroscience: to transform correlative findings in causative ones. Research of recent years has shown the tremendous potential of the central nervous system to react to noxious stimuli such as a stroke: Increased plastic changes leading to reorganization in form of neuronal rewiring, neurogenesis, and synaptogenesis, accompanied by transcriptional and translational turnover in the affected cells, have been described both clinically and in experimental stroke research. However, only minor attempts have been made to connect distinct plastic remodeling processes as causative features for specific behavioral phenotypes. Here, we review current state-of the art techniques for the examination of cortical reorganization and for the manipulation of neuronal circuits as well as techniques which combine anatomical changes with molecular profiling. We provide the principles of the techniques together with studies in experimental stroke research which have already applied the described methodology. The tools discussed are useful to close the loop from our understanding of stroke pathology to the behavioral outcome and may allow discovering new targets for therapeutic approaches. The here presented methods open up new possibilities to assess the efficiency of rehabilitative strategies by understanding their external influence for intrinsic repair mechanisms on a neurobiological basis.
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影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
DOI：
10.1098/rstb.1999.0541
发表时间：
1999-12-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Crick, F
通讯作者：
Crick, F
影响因子：
5.3
作者：
Brus-Ramer, Marcel;Carmel, Jason B.;Martin, John H.
通讯作者：
Martin, John H.
DOI：
10.1073/pnas.0906593106
发表时间：
2009-11-10
影响因子：
11.1
作者：
Guettier, Jean-Marc;Gautam, Dinesh;Wess, Juergen
通讯作者：
Wess, Juergen